Ion Exchange Chromatography Columns

All Shodex IEC columns are packed with chemically atable polymers that can be used in pH2 to 12.

For cation exchange

SP-825 CM-825	· For the analysis of high-melecular-weight molecules, such as proteins, peptides, DNA and RNA · Polyhydroxy methacrylate-based packing material supports the use in pH rangeof 2 to 12 · Semi-micro, micro and preparative columns are available
SP-420N	· Nonporous base material · Rapid analysis · Wide pH range (pH2 to12)
ES-502C 7C	· Polyvinyl alcohol-based packing material supports the use of eluent in a wide pH range (pH2 to 12) · Less hydrophobic interaction with proteins allows milder analytical conditions · Semi-micro, micro and preparative columns are available

(Standard Columns)
Strong cation echange resin (Functional group: Sulfopropyl)

Product name	Ion exchange capacity(meq/g)	Particle size (micron)	Size(mm) ID x Length	Shipping solvent
IEC SP-825 IEC SP-420N	0.4 0.3	8 2.5	8.0 x 75 4.6 x 35	50mM Na2SO4 aq. 20mM Sodium acetate buffer + 0.5M Na2SO4(pH5.0)

Product name	Max. usable pressure(MPa)	Max. usable flow rate(mL/min)	Usable temp. range(deg-C)	Usable pH range	Usable organic solvent conc.	Usable salt conc.
SP-825 SP-420N	2.0 20	1.5 2.0	10 to 50 0 to 60	2 to 12 2 to 12	20% 20%	20mM to 1.0M < 1.5M

Weak cation echange resin (Functional group: Carboxymethyl)

Product name	Ion exchange capacity(meq/g)	Particle size (micron)	Size(mm) ID x Length	Shipping solvent
IEC CM-825	0.4	8	8.0 x 75	50mM Na2SO4 aq.
Asahipak ES-502C 7C	0.55	9	7.5 x 100	0.1M Sodium phosphate buffer(pH4.4)

Product name	Max. usable pressure(MPa)	Max. usable flow rate(mL/min)	Usable temp. range(deg-C)	Usable pH range	Usable organic solvent conc.	Usable salt conc.
CM-825	2.0	1.5	10 to 50	2 to 12	20%	20mM to 1.0M
ES-502C 7C	1.2	1.5	10 to 50	2 to 12	100%	20mM to 0.6M

(Micro column and Semi-micro columns)(Remark)
Strong cation echange resin

Product name	Size(mm) ID x Length	Standard column
SP8-2D SP8-2B SP8-2T	2.0 x 150 2.0 x 50 2.0 x 35	SP-825
SP8-1D SP8-1B SP8-1T	1.0 x 150 1.0 x 50 1.0 x 35	SP-825
SP8-M8D SP8-M8B SP8-M8T	0.8 x 150 0.8 x 50 0.8 x 35	SP-825
SP8-M5D SP8-M5B SP8-M5T	0.5 x 150 0.5 x 50 0.5 x 35	SP-825
SP8-M3D SP8-M3B SP8-M3T	0.3 x 150 0.3 x 50 0.3 x 35	SP-825

Product name	Size(mm) ID x Length	Standard column
SP3N-2B SP3N-2T	2.0 x 50 2.0 x 35	SP-420N
SP3N-1B SP3N-1T	1.0 x 50 1.0 x 35	SP-420N
SP3N-M8B SP3N-M8T	0.8 x 50 0.8 x 35	SP-420N
SP3N-M5B SP3N-M5T	0.5 x 50 0.5 x 35	SP-420N
SP3N-M3B SP3N-M3T	0.3 x 50 0.3 x 35	SP-420N

Weak cation echange resin

Product name	Size(mm) ID x Length	Standard column
CM8-2D CM8-2B CM8-2T	2.0 x 150 2.0 x 50 2.0 x 35	CM8-825
CM8-1D CM8-1B CM8-1T	1.0 x 150 1.0 x 50 1.0 x 35	CM8-825
CM8-M8D CM8-M8B CM8-M8T	0.8 x 150 0.8 x 50 0.8 x 35	CM8-825
CM8-M5D CM8-M5B CM8-M5T	0.5 x 150 0.5 x 50 0.5 x 35	CM8-825
CM8-M3D CM8-M3B CM8-M3T	0.3 x 150 0.3 x 50 0.3 x 35	CM8-825

Product name	Size(mm) ID x Length	Standard column
CM9A-2D CM9A-2B CM9A-2T	2.0 x 150 2.0 x 50 2.0 x 35	ES-502C 7C
CM9A-1D CM9A-1B CM9A-1T	1.0 x 150 1.0 x 50 1.0 x 35	ES-502C 7C
CM9A-M8D CM9A-M8B CM9A-M8T	0.8 x 150 0.8 x 50 0.8 x 35	ES-502C 7C
CM9A-M5D CM9A-M5B CM9A-M5T	0.5 x 150 0.5 x 50 0.5 x 35	ES-502C 7C
CM9A-M3D CM9A-M3B CM9A-M3T	0.3 x 150 0.3 x 50 0.3 x 35	ES-502C 7C

(Preparative Columns)(Remark)
Strong cation echange resin

Product name	Particle size (micron)	Size(mm) ID x Length	Sandard column
IEC SP-2025 IEC SP-2825 IEC SP-5025 IEC SP-LG	20 37 37 20	20.0 x 150 28.0 x 300 50.0 x 300 8.0 x 50	SP-825 SP-825 SP-825 Guard column

Weak cation echange resin

Product name	Particle size (micron)	Size(mm) ID x Length	Sandard column
IEC CM-2025 IEC CM-2825 IEC CM-5025 IEC CM-LG	20 37 37 20	20.0 x 150 28.0 x 300 50.0 x 300 8.0 x 50	CM-825 CM-825 CM-825 Guard column
Asahipak ES-502C 20C Asahipak GS-20G 7B	13 20	20.0 x 100 7.5 x 50	ES-502C 7C Guard column

For anion exchange

QA-825 DEAE-825	· For the analysis of high-melecular-weight molecules, such as proteins, peptides, DNA and RNA · Polyhydroxy methacrylate-based packing material supports the use in pH rangeof 2 to 12 · Semi-micro, micro and preparative columns are available
DEAE3N-4T	· Nonporous base material · Rapid analysis · Wide pH range (pH2 to12)
ES-502N 7C	· Polyvinyl alcohol-based packing material supports the use of eluent in a wide pH range (pH2 to 12) · Less hydrophobic interaction with proteins allows milder analytical conditions · Semi-micro, micro and preparative columns are available
WA-624	· For anion exchange analyis of small molecules such as nucleotides

(Standard Columns)
Strong anion echange resin (Functional group: Quanternary ammonium)

Product name	Ion exchange capacity(meq/g)	Particle size (micron)	Size(mm) ID x Length	Shipping solvent
IEC QA-825	0.45	12	8.0 x 75	50mM Na2SO4 aq.

Product name	Max. usable pressure(MPa)	Max. usable flow rate(mL/min)	Usable temp. range(deg-C)	Usable pH range	Usable organic solvent conc.	Usable salt conc.
QA-825	2.0	1.5	10 to 50	2 to 12	20%	20mM to 1.0M

Weak anion echange resin (Functional group: Diethylaminoethyl)

Product name	Ion exchange capacity(meq/g)	Particle size (micron)	Size(mm) ID x Length	Shipping solvent
IEC DEAE-825 IEC DEAE3N-4T	0.6 0.4	8 2.5	8.0 x 75 4.6 x 35	50mM Na2SO4 aq. H2O
Asahipak ES-502N 7C	0.55	9	7.5 x 100	50mM 1,3-Diaminopropane+ 50mM NaCl(pH10.0)
AXpak WA-624 AXpak WA-G	1.2 Guard column	10 19	6.0 x 150 4.6 x 10	0.1M Sodium phosphate buffer(pH3.0)/CH3CN=80/20 0.1M Sodium phosphate buffer(pH3.0)/CH3CN=80/20

Product name	Max. usable pressure(MPa)	Max. usable flow rate(mL/min)	Usable temp. range(deg-C)	Usable pH range	Usable organic solvent conc.	Usable salt conc.
DEAE-825 DEAE3N-4T	2.0 20	1.5 2.0	10 to 50 0 to 60	2 to 12 2 to 12	20% 20%	20mM to 1.0M < 1.0M
ES-502N 7C	1.2	1.5	10 to 50	2 to 12	100%	20mM to 0.6M
WA-624 WA-G	2.5 -	1.2 -	4 to 60 -	3 to 12 -	20% -	< 1.0M < 1.0M

(Micro column and Semi-micro columns)(Remark)
Strong anion echange resin

Product name	Size(mm) ID x Length	Standard column
QA8-2D QA8-2B QA8-2T	2.0 x 150 2.0 x 50 2.0 x 35	QA-825
QA8-1D QA8-1B QA8-1T	1.0 x 150 1.0 x 50 1.0 x 35	QA-825
QA8-M8D QA8-M8B QA8-M8T	0.8 x 150 0.8 x 50 0.8 x 35	QA-825
QA8-M5D QA8-M5B QA8-M5T	0.5 x 150 0.5 x 50 0.5 x 35	QA-825
QA8-M3D QA8-M3B QA8-M3T	0.3 x 150 0.3 x 50 0.3 x 35	QA-825

Weak anion echange resin

Product name	Size(mm) ID x Length	Standard column
DEAE8-2D DEAE8-2B DEAE8-2T	2.0 x 150 2.0 x 50 2.0 x 35	DEAE8-825
DEAE8-1D DEAE8-1B DEAE8-1T	1.0 x 150 1.0 x 50 1.0 x 35	DEAE8-825
DEAE8-M8D DEAE8-M8B DEAE8-M8T	0.8 x 150 0.8 x 50 0.8 x 35	DEAE8-825
DEAE8-M5D DEAE8-M5B DEAE8-M5T	0.5 x 150 0.5 x 50 0.5 x 35	DEAE8-825
DEAE8-M3D DEAE8-M3B DEAE8-M3T	0.3 x 150 0.3 x 50 0.3 x 35	DEAE-825

Product name	Size(mm) ID x Length	Standard column
DEAE3N-2B DEAE3N-2T	2.0 x 50 2.0 x 35	DEAE3N-4T
DEAE3N-1B DEAE3N-1T	1.0 x 50 1.0 x 35	DEAE3N-4T
DEAE3N-M8B DEAE3N-M8T	0.8 x 50 0.8 x 35	DEAE3N-4T
DEAE3N-M5B DEAE3N-M5T	0.5 x 50 0.5 x 35	DEAE3N-4T
DEAE3N-M3B DEAE3N-M3T	0.3 x 50 0.3 x 35	DEAE3N-4T

Product name	Size(mm) ID x Length	Standard column
DEAE9A-2D DEAE9A-2B DEAE9A-2T	2.0 x 150 2.0 x 50 2.0 x 35	ES-502N 7C
DEAE9A-1D DEAE9A-1B DEAE9A-1T	1.0 x 150 1.0 x 50 1.0 x 35	ES-502N 7C
DEAE9A-M8D DEAE9A-M8B DEAE9A-M8T	0.8 x 150 0.8 x 50 0.8 x 35	ES-502N 7C
DEAE9A-M5D DEAE9A-M5B DEAE9A-M5T	0.5 x 150 0.5 x 50 0.5 x 35	ES-502N 7C
DEAE9A-M3D DEAE9A-M3B DEAE9A-M3T	0.3 x 150 0.3 x 50 0.3 x 35	ES-502N 7C

(Preparative Columns)(Remark)
Strong anion echange resin

Product name	Particle size (micron)	Size(mm) ID x Length	Sandard column
IEC QA-2025 IEC QA-2825 IEC QA-5025 IEC QA-LG	20 37 37 20	20.0 x 150 28.0 x 300 50.0 x 300 8.0 x 50	QA-825 QA-825 QA-825 Guard column

Weak anion echange resin

Product name	Particle size (micron)	Size(mm) ID x Length	Sandard column
IEC DEAE-2025 IEC DEAE-2825 IEC DEAE-5025 IEC DEAE-LG	20 37 37 20	20.0 x 150 28.0 x 300 50.0 x 300 8.0 x 50	DEAE-825 DEAE-825 DEAE-825 Guard column
Asahipak ES-502N 20C Asahipak GS-20G 7B	13 20	20.0 x 100 7.5 x 50	ES-502N 7C Guard column