Features of RSpak DE Series (7) (Effect of Sample Load on Column Efficiency)
Di-n-butyl ketone were analyzed using RSpak DE-413
(a column for reversed phase chromatography).
When the sample concentration was constant, a decrease of column efficiency was observed accroding to an icrease of injection volume.
The decrease started from 50μL and it was marked when the injection volume exceeded 100μL.
To the contrary, when the injection volume was constant, a decrease of column efficiency was not observed until sample
load exceeded 500μg.
Sample : Di-n-butyl ketone, 5-Nonanone
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : CH3CN/H2O=50/50
Flow rate : 1.0mL/min
Detector : UV(262nm)
Column temp. : 40°C