Characteristics and Application of Reversed Phase, Hydrophilic Interaction
and Normal Phase Chromatography Columns

RSpak RP18-415 has a pore size of approximately 450 Å and are suitable for the analysis of proteins. Asahipak ODP, C8P and C4P have a pore size of approximately 250 Å and are suitable for peptides.
RSpak DE-613 and DE-413 having small pore size can be used for the analysis of a low molecular weight substances such as oligopeptides. Should none the DE-613, 413 or ODS columns give satisfactory results due the non-retention of the sample then the use of the RSpak DS-413 or DS-613 which have a lower polarity can be recommended.