Column Test Conditions

To check the deterioration of column performance, measure the column plate number and compare it with the number written in the inspection data sheet which is attached to the column. The test conditions for calculation of the plate number is determined for each column type and shown in the table below.
When you first use a column, we recommend you to measure its plate number before use. Plate number (N) can be caluculated by the formula in the right figure.
For the columns whose test conditions are shown with blue letters, the column performance should be measured by resolution, and not plate number.

Category	Column type		Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
Normal & reversed phase (Polymer- based)	Asahipak	ODP C8P,C4P	(1)0.75micro-L/mL n-Hexyl benzoate (2) 6micro-L(ODP), 10micro-L(C8P,C4P) (3) CH3CN/H2O=65/35 (4)0.6mL/min (5)30deg-C (6)UV(254nm)
	Asahipak	NH2P	(1)5mg/mL Sucrose (2)5micro-L(4E, 4D), 2micro-L(2D)(3) CH3CN/H2O=75/25 (4)0.6mL/min(4D), 1.0mL/min(4E), 0.2mL/min(2D) (5)30deg-C (6)RI
	RSpak	RP18-415	(1)1% Acetone (2)3micro-L (3) CH3CN/H2O=95/5 (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	RSpak	RP18-613 RP18-413 DS-413	(1)1% 3-Pentanone (2)10micro-L(613), 5micro-L(413) (3) CH3CN/THF/H2O=30/30/40 (4)0.8mL/min(613), 0.6mL/min(413) (5)RT(25deg-C) (6)UV(262nm)
	RSpak	DS-613	(1)0.5% Benzene (2)5micro-L (3) CH3CN/THF/H2O=40/30/30 (4)0.8mL/min (5)RT(25deg-C) (6)UV(254nm)
	RSpak	DE-613	(1)0.2% Ethylene glycol (2)20micro-L (3) H2O (4)0.8mL/min (5)RT(25deg-C) (6)RI
	RSpak	DE-413 GOLF CARB	(1)0.6% Di-n-butyl ketone (2)10micro-L (3) CH3CN/H2O=50/50 (4)1.0mL/min (5)40deg-C (6)UV (262nm)
	RSpak	DE-213	(1)0.6% Di-n-butyl ketone (2)3micro-L (3) CH3CN/H2O=50/50 (4)0.2mL/min (5)40deg-C (6)UV (262nm)
	RSpak	DM-614	(1)2% Oxalic acid (2)5micro-L (3)0.005M H3PO4 aq. (4)0.8mL/min (5)RT(25deg-C) (6)RI
	RSpak	DC-613	(1)2% Sucrose (2)10micro-L (3)CH3CN/H2O=70/30 (4)0.8mL/min (5)RT(25deg-C) (6)RI
	RSpak	NN	(1)0.1% Uracil(414), 0.01% Uracil(614, 814) (2) 7micro-L(414, 614), 10micro-L(814) (3)0.1M NaH2PO3aq.(pH3.0 adjusted with phosphoric acid) (4)0.3mL/min(414), 0.8mL/min(614), 1.0mL/min(814) (5)35deg-C (6)RI(414), UV(260nm)(614, 814)
Normal & reversed phase (Silica- based)	ODSpak	F	(1)0.15micro-L/mL t-Butyl benzene (2)2micro-L(411/S), 20micro-L(411), 30micro-L(511) (3)Methanol/H2O=80/20 (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	Silica	C18M C18P	(1)0.61mg/mL Naphthalene (2)2micro-L(4E), 1.5micro-L(4D) (3)Methanol/H2O=70/30 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
	Silicapak	E-411	(1)0.05% p-Nitroaniline (2)5micro-L (3) IPA/Dichloromethane/n-hexane=1/50/49 (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	Silica	5SIL
	Silica	5C8,5C4 5TMS,5CN	(1)0.61mg/mL Naphthalene (2)2micro-L(4E), 1.5micro-L(4D) (3)Methanol/H2O=55/45(5C4), Methanol/H2O=66/36(5C8), Methanol/H2O=50/50(TMS), Methanol/H2O=40/60(CN) (4)1.0mL/min (5)30deg-C (6)UV(254nm)
	Silica	5NH	(1)p-Nitrophenyl-alpha-D-glucopyranoside (2)2micro-L(4E), 1.5micro-L(4D) (3)CH3CN/H2O=95/5 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
	Silica	5NPE
	Silica	5PYE
Ion exchange	AXpak	WA-624	(1)0.03% Uridine 5-monophosphate(UMP) (2)10micro-L (3)0.1M NaH2PO3(pH3.0 adjusted with phosphoric acid)/CH3CN=80/20 (4)1.0mL/min (5)RT(25deg-C) (6)UV(260nm)
	CXpak	P-421S	(1)1% Ethylene glycol (2)10micro-L (3)H2O (4)0.5mL/min (5)RT(25deg-C) (6)RI
	IEC	QA,DEAE SP,CM	(1)0.5% Acetone (2)20micro-L (3)50mM Sodium sulfate aq. (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
	IEC	DEAE-N	(1)Oligodeoxyadenylic acid (pd(A)7) (A) 0.01unit/2micro-L (2)2micro-L (3)50mM phosphoric acid buffer(pH6.0) + 0.17M NaCl + 1% Methanol (4)1.0mL/min (5)30deg-C (6)UV(260nm)
	IEC	SP-N	(1)0.2% Glycyl-L-tyrosine (2)1micro-L (3)20mM Acetate buffer(pH5.0) + 0.5M Sodium sulfate (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
	Asahipak	ES-502N	(1)0.025% Xanthine (2)15micro-L (3)50mM 1,3 Diaminopropane + 50mM NaCl(pH10.0) (4)1.0mL/min (5)30deg-C (6)UV detector(250nm)
	Asahipak	ES-502C	(1)0.025% Cytosine (2)15micro-L (3)100mM Sodium phosphate buffer(pH4.4) (4)1.0mL/min (5)30deg-C (6)UV(250nm)
Hydrophobic chromato- graphy	HIC	PH-814	(1)0.5% Acetone (2)20micro-L (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
Affinity chromato- graphy	AFpak	32 kinds
Sugar analysis	SUGAR	SH1011 SH1821	(1)1% Glycerol (2)10micro-L (3)0.005mM H2SO4 aq. (4)1.0mL/min (5)50deg-C (6)RI
	SUGAR	SP0810	(1)1% Glycerol (2)10micro-L (3)H2O (4)0.8mL/min (5)80deg-C (6)RI
	SUGAR	SC1011 SC1811	(1)1% Glycerol (2)10micro-L (3)H2O (4)1.0mL/min (5)80deg-C (6)RI
	SUGAR	SC1211	(1)1% Glycerol (2)10micro-L (3)CH3CN/H2O=25/75 (4)1.0mL/min (5)50deg-C (6)RI
	SUGAR	SZ5532	(1)1% Glucose (2)10micro-L (3)CH3CN/H2O=70/30 (4)1.0mL/min (5)50deg-C (6)RI
	SUGAR	KS-800	(1)3% Ethylene glycol(801), 2.5% Ethylene glycol(802), 0.5% Ethylene glycol(803), 0.8% Glucose(804 to 806), 0.5% Glucose(807) (2)5micro-L(801 to 802), 15micro-L(803), 20micro-L(804,805),10micro-L(806), 5micro-L(807) (3)H2O (4)1.0mL/min (5)50deg-C(801 to 806), RT(25deg-C)(807) (6)RI
	USPpak	MN	(1)0.5% Mannitol (2)10micro-L (3)H2O (4)1.0mL/min (5)60deg-C (6)RI
Organic acid analysis	RSpak	KC-811	(1)3% Acetic acid (2)5micro-L (3)0.1%H2SO4 aq. (4)1.0mL/min (5)40deg-C (6)RI
Chiral separation	ORpak	CDA-453	(1)0.1% 6-fluoro-DL-tryptophan (2)10micro-L (3)H2O (4)0.3mL/min (5)20deg-C (6)UV(254nm)
	ORpak	CDB-453	(1)0.1% DL-Methionine beta-naphthylamide (2)10micro-L (3)1.7% TEA/Acetic acid(pH4.0) + 0.1M NaCl in 20% CH3CN + 80% H2O (4)0.2mL/min (5)20deg-C (6)UV(254nm)
	ORpak	CDC-453	(1)0.02% Dancyl-DL-leucine (2)20micro-L (3)1% Acetic acid + 0.2M NaCl in10% CH3CN + 90% H2O (4)0.3mL/min (5)RT(25deg-C) (6)UV(254nm)
	ORpak	CDBS	(1)0.1% Nefopam-HCl (Sigma N-0391) (2)10micro-L (3)CH3CN/(1% Acetic acid + 0.2M NaCl) =30/70 (4)0.5mL/min (5)RT(25deg-C) (6)UV(254nm)
	ORpak	CRX-853	(1)0.1% R,S-Mandelic acid (2)20micro-L (3)0.25mM Copper sulfate aq. (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
GFC (Aqueous GPC)	OHpak	SB-800 HQ	(1)1% Ethylene glycol(802), 0.5% Ethylene glycol(802.5 to 806) (2)10micro-L(802), 15micro-L(802.5 to 806) (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)RI
	PROTEIN	KW-800	(1)0.2% Ethylene glycol (2)40micro-L (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)RI
Multimode	Asahipak	GS-HQ	(1)1% Ethylene glycol (2)30micro-L (3)H2O (4)0.6mL/min (5)30deg-C (6)RI
Multisolvent GPC	Asahipak	GF-HQ
Organic GPC	GPC	KF-800	(1)0.1% Propylbenzene(801 to 803), 0.05% Propylbenzene(804 to 807) (2)20micro-L(801 to 803), 40micro-L(804 to 807) (3)THF (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	KF-600	(1)0.5% Propylbenzene(601), 0.05% Phthalic acid dicyclohexyl ester(602 to 604), 0.1% Phthalic acid dicyclohexyl ester(605 to 606) (2)5micro-L (3)THF (4)0.5mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	KF-400HQ	(1)0.5% Propylbenzene(401402 to 406L) (2)5micro-L (3)THF (4)0.3mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	LF	(1)0.1% Propylbenzene(804), 0.1% Phthalic acid dicyclohexyl ester(604, 404) (2)20micro-L(804), 5micro-L(604, 404) (3)THF (4)1.0mL/min(804), 0.5mL/min(604), 0.3mL/min(404) (5)RT(25deg-C) (6)UV(254nm)
	GPC	K-800	(1)0.1% Propylbenzene(801 to 803), 0.05% Propylbenzene(804 to 807) (2)20micro-L(801 to 803), 40micro-L(804 to 807) (3)THF (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	KD-800	(1)1.0 % Acetone(801 to 804), 0.5 % Acetone(805 to 807)(2)20micro-L(801 to 804), 30micro-L(805,806), 40micro-L(807) (3)DMF (4)1.0mL/min (5)RT(25deg-C) (6)RI
	GPC	HFIP-800	(1)0.5% Acetone (2)30micro-L (3)HFIP (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
	GPC	HT, UT	(1)10% Monochlorobenzene toluene solution (2)10micro-L (3)Toluene (4)1.0mL/min (5)RT(25deg-C) (6)RI
	GPC	AT-806MS	(1)1% Acetone (2)20micro-L (3)Toluene (4)1.0mL/min (5)RT(25deg-C) (6)RI
Ion chroato- graphy	IC	I-524A	(1)10ppm NO3- (2)30micro-L (3)2.5mM Phthalic acid(pH4.0) (4)1.5mL/min (5)40deg-C (6)CD
	IC	NI-424	(1)10ppm SO42- (2)50micro-L (3)8mM p-Hydroxybenzoic acid + 2.8mM Bis(2hydroxyethyl)imino-tris(hydroxymethyl)methan + 2mM Phenylboronic acid + 5micro-M trans-1,2-Aminocyclohexane-N,N,N',N'-4 acetic acid aq.(4)1.0mL/min (5)40deg-C (6)CD
	IC	SI-90	(1)15ppm SO42- (2)20micro-L (3)1.8mM Na2CO3 + 1.7mM NaHCO3 aq. (4)1.0mL/min (5)RT(25deg-C) (6)Ssuppressed CD
	IC	SI-50	(1)10ppm Br- (2)20micro-L (3)3.2mM Na2CO3 + 1.0mM NaHCO3 aq. (4)0.7mL/min (5)RT(25deg-C) (6)Ssuppressed CD
	IC	SI-52	(1)40ppm SO4- (2)50micro-L (3)3.6mM Na2CO3 aq. (4)0.8mL/min (5)45deg-C (6)Ssuppressed CD
	IC	T-521	(1)20ppm Na+ (2)20micro-L (3)3mM Nitric acid aq. (4)1.0mL/min (5)40deg-C (6)CD
	IC	R	(1)1% Ethylene glycol (2)2micro-L (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)RI
	IC	Y-521	(1)20ppm Na+ (2)50micro-L (3)4mM Nitric acid aq. (4)1.5mL/min (5)40deg-C (6)CD
	IC	YK-421	(1)20ppm Ca2+ (2)15micro-L (3)5mM Tartaric acid + 1mM Dipicolinic acid + 24mM Boric acid aq.(4)1.0mL/min (5)40deg-C (6)CD