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  • HPLC Lecture Notes

HPLC Lecture Notes

  1. Chapter 1: Introduction

    1. 1.History of Chromatography
    2. 2.What is an HPLC?
    3. 3.Features of HPLC
    4. 4.HPLC System Components

  2. Chapter 2: HPLC Separation

    1. 5.Principles of Separation
    2. 6.Chromatogram
    3. 7.Basic HPLC Terminology
    4. 8.HPLC Columns

  3. Chapter 3: Separation Modes and their Mechanisms (1)

    1. 9.Types of Separation Modes
    2. 10.Separation by Polarity
    3. 11.Separation by Ionic Differences

  4. Chapter 4: Separation Modes and their Mechanisms (2)

    1. 12.Separation by Ligand Exchange Ability Differences
    2. 13.Separation by Molecular Size (Molecular Weight) Differences
    3. 14.Separation by Specificity Differences

  5. Chapter 5: Detector Unit

    1. 15.Detectors for HPLC