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HPLC Lecture Notes

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  • Lesson 1: Introduction to HPLC
    1. Introduction
    2. What is Liquid Chromatography?
    3. What is HPLC?
    4. Components of HPLC
    5. Other Chromatography

  • Lesson 2: Theory and Types of HPLC Column
    1. Theory of LC Column Separation
    2. HPLC Separation
    3. Types of Packed Gels
    4. Types of Separation Mode

  • Lesson 3: Partition/Adsorption Chromatography Columns
    1. Reversed-Phase Column
    2. Normal-Phase Column
    3. Hydrophilic Interaction Chromatography Column
    4. Summary of Shodex Partition/Adsorption Columns

  • Lesson 4: SEC Columns
    1. Size Exclusion Chromatography column
    2. Gel Filtration Chromatography
    3. Gel Permeation Chromatography
    4. Linear Column
    5. Downsized Column
    6. Analysis of Polymers that require Special Conditions
    7. Multi-Mode Column
    8. Multi-Solvent Column

  • Lesson 5: Other Separation Modes, Preparative and Guard Columns
    1. Ion Exchange Column
    2. Ion Chromatography Column
    3. Ion Exclusion Column
    4. Ligand Exchange Column
    5. Affinity Column
    6. Chiral Column
    7. Preparative Size Column
    8. Guard Column

  • Lesson 6: Detectors for HPLC
    1. UV, VIS, and PDA Detectors
    2. Refractive-Index Detector
    3. Evaporative Light Scattering Detector
    4. Multi-Angle Light Scattering Detector
    5. Mass Spectrometer
    6. Conductivity Detector
    7. Fluorescence Detector
    8. Chemiluminescence Detector
    9. Optical Rotation Detector
    10. Electro Chemical Detector