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PITTCON 2018

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Data February 26 - March 1, 2018
Venue Orange County Convention Center (USA)
Official URL http://2018-pittcon.org/

 

Poster presentations

Title (960-1) Rapid Analysis of Low Molecular Weight to High Molecular Weight Polymers by Novel GPC Columns
Abstract Conventional GPC analysis has the ongoing issue of long analysis time and high-volume solvent usage. In order to solve these problems, Shodex introduced a rapid analysis GPC column, GPC HK-404L, using a porous cross-linked styrene-divinylbenzene polymer gel with sharp particle size distribution. The HK-404L can be used for molecular weight measurement with the range from 100 to 1 million Daltons. We have recently developed two new columns, one for lower molecular weight oligomers analysis in the molecular weight range from 100 to 1,500 Daltons and another column for higher molecular weight polymer with molecular weight from 10,000 to 2,500,000 Daltons. In both cases, the elution volume with less than 2.0 mL. Additionally, basic features of these novel GPC columns and several cases of molecular weight measurement will be presented.
Presenters LEAH BLOCK, Shodex, Showa Denko America
Presentation
date
February 27, 2018
10:00 - 12:00
Location Exposition Floor, Aisles 2000-2700

 

Title (1320-5) LC/MS Analysis of Oligonucleotides Using New Polymer-Based HILIC Column Having Diol Group
Abstract Highly sensitive and highly selective LC/MS analysis is required for the development or quality control of oligonucleotide drugs. Conventionally ion-pair reverse-phase chromatography have been used, unfortunately ion-pair reagents tend to remain in the LC/MS instrument. In order to solve this issue, a new HILIC column with polyvinyl alcohol base packing material modified with diol group, was used for the LC. Aqueous solutions including ammonium formate and acetonitrile were used as the eluent, coupled with ESI-MS were used for detection. As a result, good separation of 2-20mer DNA was obtained allowing the calibration curve of 20mer to show high linearity. This method enables the analysis of oligonucleotides without ion-pair reagent. This HILIC column is useful for the LC/MS analysis of oligonucleotide medicines using more simple conditions than previous methods.
Presenters LEAH BLOCK, Shodex, Showa Denko America
Presentation
date
February 28, 2018
10:00 - 12:00
Location Exposition Floor, Aisles 2000-2700

 

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