The ratio of deproteinization with MSpak GF-4A (a column for Column switching) and with a corresponding column of other company were compared. Since the ratio of deproteinazation is high, GF-2A can suppress the adsorption of protein to analytical columns or LC/MS as much as possible, and keep the reproducibility of analysis. (GF-2A (2.0 mm I.D. x 10 mm) is a custom-made column of GF-4A.)
Sample: 100 μL
BSA 200 mg/mL
Ratio of deproteinization
| MSpak GF-2A | Company A |
|---|---|
| 99.80% | 97.68% |
Pretreatment
- Columns
- ：Shodex MSpak GF-2A, Company A (2.0 mm I.D. x 50 mm each)
- Eluent
- ：10 mM CH3COONH4 (pH7.0)
- Flow rate
- ：0.5 mL/min
- Column temp.
- ：Room temp.
- Switching time
- ：5 min
Analysis
- Column
- ：Shodex PROTEIN *KW-604S (6.0 mm I.D. x 50 mm)
*KW-604S is phase-out product. - Eluent
- ：0.1 % TFA in (H2O/CH3CN=50/50)
- Flow rate
- ：0.5 mL/min
- Detector
- ：UV (280 nm)
- Column temp.
- ：Room temp.
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.