Main separation mode for the GPC clean-up is size exclusion mode: based on analytes' size differences, analytes are eluted in the order of their sizes. However, by optimizing the eluent composition, partition/adsorption mode can be also used. Change in acetone and cyclohexane ratio does not influence elution volume of lipid much. However by decreasing acetone, retention time of pesticides increases. At acetone/cyclohexane=3/7, lipid and acrinathirin co-elutes. In Food Sanitation Law #1003001 (Released by Ministry of Health, labour and welfare in Japan, 2006), the ratio of acetone/cyclohexane is set at 1/4.
Sample : 40 mg/L each, 5 mL
1. Acrinathrin
2. Tricyclazole
Columns : Shodex CLNpak EV-G AC (20.0 mm I.D. x 100 mm) + EV-2000 AC (20.0 mm I.D. x 300 mm) Eluent : Acetone/Cyclohexane Flow rate : 5 mL/min Detector : UV (254 nm) Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Profile for Pesticides (EV-2000 AC)
- Effects of Eluent Components
- Separation with Acetone/Cyclohexane Eluent (EV-2000 AC)
- Separation with Ethyl Acetate/Cyclohexane Eluent (EV-2000)
- Spinach Pigment Removal (EV-2000 AC)
- Spinach Pigment Removal - Removal Rate (EV-2000 AC)
- Effects of Sample Injection Volume
- Effects of Flow Rate (EV-2000 AC)
- Effects of Column Temperature
- Preparation of Test Samples for Agricultural Chemicals in Livestock and Marine Products
- GPC Clean-up of Pesticide Residues (EV-2000 AC)
- Fractionation Standards (EV-2000 AC)
- Elution Volume for Lipid and Fatty Acids (EV-2000 AC)
- Effects of Temperature on GPC Clean-up
- GPC Clean-up Sample (1) (Fatty Tuna and Beef Tallow)
- GPC Clean-up Sample (2) (Milk and Egg)
- Preparation Range of Agricultural Chemicals (EV2000AC-12F)