Deoxynivalenol(DON) and Nivalenol(NIV) in grains were analyzed with Silica C18M 4E after the sample pretreatment.


Sample : 20 μL
(A) Standard solution (The amount of DON and NIV are 10 ng each.)
(B) Sample solution of Wheat spiked with DON and NIV at 1 μg/mL each
Nivalenol (NIV)
Deoxynivalenol (DON)

Column       : Shodex Silica C18M 4E (4.6 mm I.D. x 250 mm)
Eluent       : H2O/CH3OH/CH3CN=90/5/5
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : 40 °C