Anthocyanidins are polyphenols found in grape skins and blueberries. They have antioxidant and anti-inflammatory effects, which are expected to improve vision and nerve health. In this application, we performed separation of six major anthocyanidins using Silica C18 M 4D, a silica-based reversed-phase chromatography column. Six anthocyanidins were analyzed within 20 minutes using an isocratic elution.

Sample: 10 μL

1. 1.Delphinidin 20 μg/mL
2. 2.Cyanidin 10 μg/mL
3. 3.Petunidin 40 μg/mL
4. 4.Pelargonidin 10 μg/mL
5. 5.Peonidin 20 μg/mL
6. 6.Malvidin 20 μg/mL

Dissolved in 1 % TFA/CH₃OH=30/70