Hexamethylenetetramine is used for various applications such as curing accelerators for thermosetting resins and raw materials for pharmaceuticals. However, when river water containing hexamethylenetetramine is chlorinated at a water treatment plant, it is known to change into toxic formaldehyde. Therefore a highly sensitive analytical method for hexamethylenetetramine is required. As hexamethylenetetramine has high polarity, retention is weak in reverse phase mode, and traditional methods require derivatization or the use of ion pair agents. HILICpak VC-50 2D is suitable for the retention of highly polar cationic substances and here, we confirmed that it is possible to analyze hexamethylenetetramine without derivatization and ion pair agents with VC-50 2D column at ppb (ng/mL) levels with LC/MS.
Sample : 10 μL
1, 5, 10 ng/mL (in H2O)
1. Hexamethylenetetramine
Column : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm) Eluent : (A) 100mM HCOOH aq./(B) CH3CN = 60/40 Flow rate : 0.25 mL/min Detector : ESI-MS (SIM Positive) Column temp. : 40 °C
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Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
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Applications
- Mild Hydrolysates of Allosamidin (ES-502C 7C)
- 5-(Hydroxymethyl)Furfural in Fruit Juice (ODP-50 6D)
- Cyanate and Carbamyl Phosphate (ES-502N 7C)
- Hydrogen Peroxide (KS-801)
- Chelating Reagents (1) (I-524A)
- Chelating Reagents (2) (NI-424)
- Analysis of Chelating Agents (ODP-50 4D)
- Hydantoins (DE-613)
- Amides (2) (Comparison of Reversed Phase Columns)
- Amides (3) (DE-413)
- Amides (4) (DE-413)
- Amides (5) (DE-413)
- Aldehydes (DE-413)
- Analysis of Humic Substances by SEC (SB-805 HQ)
- DNPH Derivative Aldehydes (ODP-50 4D)
- Analysis of Pochonicine and Its Analogues in Filamentous Fungi Culture Extract (ES-502C 7C)
- Separation of Furfural and Guaiacol (DE-413)
- Separation of C1 Compounds (DE-413)
- Simultaneous Analysis of Various Nitrogen Compounds According to Japanese Testing Methods for Fertilizers (ES-502C 7C)
- Separation of Substances Produced During Utilization of Biomass (VN-50 4D)