In Japan, the amount of various nitrogen compounds permitted in fertilizer is determined by the fertilizer control law. In this application, five kinds of nitrogen compounds were analyzed simultaneously using Asahipak ES-502C 7C, a polymer-based column for cation exchange chromatography described in the Japanese regulation for "Testing Methods for Fertilizers (2016*)". It was confirmed that the calibration curve of sulfamic acid shows good linearity respectively in the range from 1 to 50 μg/mL.

*The version at the time of the application acquisition.


Sample : 10 µg/mL each, 10 μL
1. Urea
2. Biuret
3. Dicyandiamide
4. Guanidine
5. Guanylurea

Column       : Shodex Asahipak ES-502C 7C (7.5 mm I.D. x 100 mm)
Eluent       : 3.92 g KH2PO4 + 0.12 g H3PO4 in 1000 mL of H2O
Flow rate    : 0.6 mL/min
Detector     : UV (190 nm)
Column temp. : 40 °C