Cyanate and Carbamyl Phosphate (ES-502N 7C)

Cyanate and carbamyl phosphate were analyzed using Asahipak ES-502N 7C (a column for weak cation exchange chromatography).


Sample : 20 μM each, 50 μL
1. Carbamyl phosphate
2. Cyanate



Column       : Shodex Asahipak ES-502N 7C (7.5 mm I.D. x 100 mm)
Eluent       : 0.15 M NaCl aq.
Reagent      : (I); 0.1 M acetate buffer (pH3.0) containintg 2 %(w/v) Phenol 
                    and 10 mg/dL Sodium nitropreusside 
               (II); 1.0 %(w/v) Sodium hydroxide containing 0.1 %(w/v) Sodium hypochlorite
                (for hydrolysis under acidic condition/indophenol reaction)
Flow rate    : 1.0 mL/min
Detector     : VIS (635 nm)
Column temp. : 20 °C

I.Koshiishi et al., Anal.Sci., 2 (1986) 81.



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