5-(Hydroxymethyl)furfural (5-HMF) in fruit juice was analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography.


Sample : 5-(Hydroxymethyl)furfural (5-HMF) in Fruit juice
100% Natural orange juice
100% Natural grape juice

Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH3.0)/CH3CN=90/10
Flow rate    : 1.0 mL/min
Detector     : UV (283 nm)
Column temp. : 50 °C