LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)

Carnitine and Trimethyl glycine were analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for high sensitivity analysis by LC/MS.
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Sample : 5 μL
5 ng/mL each
(in H2O/CH3CN=25/75)
1. Trimethyl glycine
2. Carnitine


Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : 50 mM HCOONH4 aq./CH3CN=20/80
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Positive)
Column temp. : 30 °C

Sample Name Index

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