Phenols were analyzed using RSpak DE-413, a column for reversed phase chromatography.


Sample : 5 μL
(Fluorescence) 1 μg/mL each
(UV) 100 μg/mL each
1. Phenol
2. 4-tert-Butylphenol
3. 4-Octylphenol
4. Nonylphenol


Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : CH3CN/H2O=70/30
Flow rate    : 1.0 mL/min
Detector     : Fluorescence (Ex. 225 nm, Em. 300 nm), UV (220 nm)
Column temp. : 40 °C

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