Preservatives (4) (DE-413)

Food aditives, which have wide polarity range, can be analyzed using RSpak DE-413 (a column for reversed phase chromatography) by simple isocratic mode in a short time.


Sample : 10 μL
1. Saccharin sodium 0.005 %
2. p-Hydroxybenzoic acid 0.005 %
3. Sorbic acid 0.02 %
4. Benzoic acid 0.02 %
5. Methyl paraben, Methyl p-hydroxybenzoate 0.01 %
6. Dehydroacetic acid 0.01 %
7. Ethyl paraben, Ethyl p-hydroxybenzoate 0.02 %
8. Propyl paraben, Propyl p-hydroxybenzoate 0.01 %

Column       : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent       : (0.05 M KH2PO4 + 0.1 % H3PO4) aq./CH3CN=35/65
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C

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Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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