Benzoates were analyzed using RSpak DE-413, a column for reversed phase chromatography.

Sample:

  1. 1.Methyl benzoate
  2. 2.Butyl benzoate
  3. 3.Hexyl benzoate
Column
Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent
(left); CH3CN/H2O=65/35, (center); CH3OH/H2O=80/20, (right); THF/H2O=50/50
Flow rate
0.6 mL/min
Detector
UV (254 nm)
Column temp.
40 ℃

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