For the analysis of nonylphenol ethoxylate using Asahipak GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column), most of organic solvents can be used.
The figure shows the elution characteristics of nonylphenol ethoxylate when typical organic solvents are used. The elution time of nonylphenol ethoxylate is the fastest when n-propanol is used, medium when acetonitrile is used and the latest when methanol is used. The order is as same as the case when reversed phase columns are used.


Sample :
Nonylphenol ethoxylate (n=20)

Column       : Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm)
Flow rate    : 0.6 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C