Sulfurous acid is a weak acid and can be separated by ion exclusion chromatography using RSpak KC-811. (A) is a chromatogram of standards. (B) is a chromatogram of directly injection of diluted wine and only free sulfurous acid is detected. (C) is a chromatogram of diluted wine to which NaOH is added before injection. By the addition of NaOH, all sulfurous acid in the wine is converted to free sulfurous acid and the amount of total sulfurous acid can be analyzed.
Sample : (B), (C); Wine
1. SO32-
2. Uric acid
Column : Shodex RSpak KC-811 (8.0 mm I.D. x 300 mm) Eluent : 12 mM H3PO4 aq. Flow rate : 1.2 mL/min Detector : ECD (Electrode;Pt, 600 mV SCE) Column temp. : 40 °C
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Applications
- Separation of Nitrite and Nitrate Ions (NH2P-50 4E)
- Sulfite in Wine (1) (KC-811)
- Silicic Acid and Boric Acid (KC-811)
- Arsenic Acid and Arsenious Acid (KC-811)
- Simultaneous Analysis of Carbonic Ion and Seven Common Anions (SI-90 4E)
- Effects of Pretreatment in The Analysis of Mineral Water (SI-90 4E)
- Simultaneous Analysis of Nitrous Acid and Ammonium Thiocyanate According to Japanese Testing Methods for Fertilizers (NH2P-50 4E)
- Separation of Water-Soluble Vitamin and Sulfite (KC-811)