Trialkylammonium were separated using cation chromatography column, IC YK-421 . By using the mobile phase used below (high ACN concentration), hydrophobic interaction is suppressed. Thus ioninc-interaction dominates, this makes shorter chain alkylammonium to be retained longer than longer ones. 
Sample : 100 mg/L each, 20 μL
1. Tetrahexylammonium
2. Tetrapentylammonium
3. Tetrabutylammonium
4. Tetrapropylammonium
5. Tetraethylammonium
6. Tetramethylammonium
Column : Shodex IC YK-421 (4.6 mm I.D. x 125 mm) Eluent : 2.5 mM HNO3 aq./CH3CN=30/70 Flow rate : 1.0 mL/min Detector : Non-suppressed coductivity Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Monovalent and Divalent Cation Standards (1) (YK-421)
- Monovalent and Divalent Cation Standards (2) (YK-421)
- Monovalent and Divalent Cation Standards (3) (Dipicolinic Acid + Crown Ether Eluent) (YK-421)
- Monovalent and Divalent Cation Standards (4) (YK-421) (Phosphoric Acid Eluent)
- Monovalent and Divalent Cation Standards (5) (YK-421) (Phosphoric Acid + 18-Crown 6-Ether Eluent)
- Monovalent and Divalent Cation Standards (6) (Comparison of YS-50 and YK-421)
- Monovalent and Divalent Cation Standards (7) (Eluent Conteinig Acetonitrile) (YS-50)
- Monovalent and Divalent Cation Standards (8) (Ethylenediamine Containing Sample) (YS-50)
- Dynamic Range of Column (YK-421)
- High Sensitive Detection of Cations (YK-421)
- Effects of Acetonitrile Concentration on Elution Volume (YK-421)
- Effects of Methanesulfonic Acid Concentration on Elution Time (YS-50)
- Effects of Flow Rate on Plate Number (YS-50)
- Effects of Temperature (YS-50)
- Effects of Sample Load on Plate Number (YS-50)
- Linearity of Calibration Curves (YS-50)
- Effects of Crown Ethers (YS-50)
- Tap Water (1) (YK-421)
- Analysis of Tap Water (2) (YK-412) (Phosphoric Acid Eluent)
- Monovalent and Divalent Cations Analysis in Tap Water (Ethylenediamine Containing Sample) (YS-50)
- Monovalent and Divalent Cations Analysis in River Water (Ethylenediamine Containing Sample) (YS-50)
- Mineral Water (YS-50)
- Sea Water (2) (YS-50)
- Red Wine (1) (YK-421)
- Red Wine (3) (YS-50)
- Serum (YK-421)
- Long Chain Alkylamines (YK-421)
- Trialkylamines (YK-421)
- Analysis of Tetraalkylammoniums (YS-50)
- Alkylamines (2) (YK-421) (Phosphoric Acid + Acetonitrile)
- Ethanolamines and Alkylamines (YK-421) (Phosphoric Acid + Acetonitrile)
- Analysis of Ethanolamines and Alkylamines (YS-50)
- Cations and Alkylamines (YS-50)
- Analysis of Methylamines (YS-50)
- Analysis of Ethylamines (YS-50)
- Analysis of Short Chain Alkylamines (YS-50)
- Cation Analysis in the Presence of PEG (YS-50)
- Cation Analysis in the Presence of Albumin (YS-50)
- Quantification of N-Methylpyrrolidine in Cefepime for Injection According to USP-NF Method (YK-421)