The problems in the anaysis using IC YK-421 is that the analysis of very small amount of NH 4 + in a sample which contains large amount of Na + is difficult. Addition of 2.5 mM 18-Crown 6-ether in 5 mM Tartaric acid + 1 mM Dipicolinic acid + 1.5 g/L Boric acid eluent can solve the problem. Addition of crown 6-ether can adjust the elution volume of the cations. Especially, the elution volume of K + is largely affected by the additon and elute after Mg 2+ .


Sample : Serum, 10 μL
1. Na +
2. NH 4 +
3. Ca 2+
4. Mg 2+
5. K +
 
Column       : Shodex IC YK-421 (4.6 mm I.D. x 125 mm)
Eluent       : (A) 5 mM Tartaric acid + 1 mM Dipicolinic acid + 1.5 g/L Boric acid aq.
               (B) 5 mM Tartaric acid + 1 mM Dipicolinic acid + 1.5 g/L Boric acid
                   + 2.5 mM 18-Crown 6-ether aq.
Flow rate    : 1.0 mL/min
Detector     : Non-suppressed conductivity
Column temp. : 40 °C

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