1. 5 mM tartaric acid + 1 mM dipicolinic acid Eluent + 1.5 g/L boric acid
The eluent of 5 mM tartaric acid + 1 mM dipicolinic acid + 1.5 g/L boric acid is the one generally used for the analysis of cations such as Na+, NH4+, K+, Ca2+ and Mg2+ which are most commonly contained in actual samples. Although Mn2+ elute just after K+, all the other transition metal ions elute at Vo.
Separation can be adjusted by changing the eluent concentration as follows :
* Tartaric acid------5 mM is used most commonly. The use of higher concentrations such as 6 mM to 7 mM, causes cations to elute faster.
* Dipicolinic acid---1 mM is used most commonly. It is added to make divalent cations elute faster.
At a higher temperature such as 50 °C, Sr2+, Ba2+ elute faster.
(Preparation of eluent)
1) Put 0.750 g of reagent grade L(+)tartaric acid, 0.167 g of reagent grade dipicolinic acid and 1.5 g of reagent grade boric acid into a volumetric flask.
2) Dilute with purified water to make up a 1 liter solution.
3) Dissolve applying ultrasonic vibration.
4) Pass through a 0.2 μm membrane filter before use.
(Remark) Since tartaric acid deteriorates so fast, this eluent should be prepared just before the use.
2. Addition of crown ether
The separation between Na+ and NH4+ can be largely improved by adding crown ether in the eluent of 5 mM tartaric acid + 1 mM dipicolinic acid + 1.5 g/L boric acid.
For example, Na+ and NH4+ in serum of which concentration ratio is 1 : 300 can be separated well.
3. Addition of acetonitrile
Acetonitrile can be added up to 70 % in nitric acid aqueous solution. Therefore, in addition to organic cations, ethanolamine and short chain alkylamines which could be analysed using the old version column(YF-421), long chain alkylamines can also be analysed using IC YK-421.
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Monovalent and Divalent Cation Standards (1) (YK-421)
- Monovalent and Divalent Cation Standards (2) (YK-421)
- Monovalent and Divalent Cation Standards (3) (Dipicolinic Acid + Crown Ether Eluent) (YK-421)
- Monovalent and Divalent Cation Standards (4) (YK-421) (Phosphoric Acid Eluent)
- Monovalent and Divalent Cation Standards (5) (YK-421) (Phosphoric Acid + 18-Crown 6-Ether Eluent)
- Monovalent and Divalent Cation Standards (6) (Comparison of YS-50 and YK-421)
- Monovalent and Divalent Cation Standards (7) (Eluent Conteinig Acetonitrile) (YS-50)
- Monovalent and Divalent Cation Standards (8) (Ethylenediamine Containing Sample) (YS-50)
- Dynamic Range of Column (YK-421)
- High Sensitive Detection of Cations (YK-421)
- Effects of Acetonitrile Concentration on Elution Volume (YK-421)
- Effects of Methanesulfonic Acid Concentration on Elution Time (YS-50)
- Effects of Flow Rate on Plate Number (YS-50)
- Effects of Temperature (YS-50)
- Effects of Sample Load on Plate Number (YS-50)
- Linearity of Calibration Curves (YS-50)
- Effects of Crown Ethers (YS-50)
- Tap Water (1) (YK-421)
- Analysis of Tap Water (2) (YK-412) (Phosphoric Acid Eluent)
- Monovalent and Divalent Cations Analysis in Tap Water (Ethylenediamine Containing Sample) (YS-50)
- Monovalent and Divalent Cations Analysis in River Water (Ethylenediamine Containing Sample) (YS-50)
- Mineral Water (YS-50)
- Sea Water (2) (YS-50)
- Red Wine (1) (YK-421)
- Red Wine (3) (YS-50)
- Serum (YK-421)
- Long Chain Alkylamines (YK-421)
- Trialkylamines (YK-421)
- Tetraalkylammoniums (YK-421)
- Analysis of Tetraalkylammoniums (YS-50)
- Alkylamines (2) (YK-421) (Phosphoric Acid + Acetonitrile)
- Ethanolamines and Alkylamines (YK-421) (Phosphoric Acid + Acetonitrile)
- Analysis of Ethanolamines and Alkylamines (YS-50)
- Cations and Alkylamines (YS-50)
- Analysis of Methylamines (YS-50)
- Analysis of Ethylamines (YS-50)
- Analysis of Short Chain Alkylamines (YS-50)
- Cation Analysis in the Presence of PEG (YS-50)
- Cation Analysis in the Presence of Albumin (YS-50)
- Quantification of N-Methylpyrrolidine in Cefepime for Injection According to USP-NF Method (YK-421)