Colominic acid is a homopolymer composed of α (2-8)-linked N-acetyl-D-neuraminic acid residues. In this application, colominic acid was analyzed using OHpak SB-804 HQ, an aqueous SEC (GFC) column. Water and basic aqueous solution were used as the eluent. Although a normal distribution chromatogram was obtained under the condition of basic aqueous solution, a part of colominic acid eluted at the exclusion volume due to ionic interaction between the packing material under the condition of water. The basic aqueous solution may suppress the ionic interactions between the sample and the packing material in the analysis of acidic polymer such as colominic acid.
Sample : 50 µL
Colominic acid sodium salt
from Escherichia coli 0.1 %(w/v)
Column : Shodex OHpak SB-804 HQ (8.0 mm I.D. x 300 mm) Flow rate : 1.0 mL/min Detector : RI Column temp. : 30 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Pullulan Standards (1) (SB-804 HQ)
- Pullulan Standards (2) (GF-310 HQ)
- Pullulan Standards (4) (Effects of Flow Rate) (SB-2002.5)
- Pullulan Standards (5) (Effects of Temperature) (SB-2002.5)
- Pullulan Standards (7) (GS-220 HQ)
- Pullulan Standards (9) (GF-7M HQ)
- Poly(Ethylene Oxide) and Poly(Ethylene Glycol) Standards (SB-804 HQ)
- Dextran (1) (SB-805 HQ)
- Dextran (2) (DMSO Eluent) (KF-806M)
- Dextran (3) (SB-806M HQ)
- Polyacrylamide (1) (SB-805 HQ)
- Polyacrylamide (3) (Comparison with SB-807 HQ and SB-806 HQ)
- Poly(Vinyl Alcohol) (2) (Aqueous Eluent) (SB-806 HQ + SB-803 HQ)
- Sodium Polyacrylate (1) (SB-803 HQ + SB-802 HQ)
- Optimization of Mobile Phase for the Analysis of Sodium Polyacrylate (GF-7M HQ)
- Polyvinylpyrrolidone (3) (NaCl aq./CH3CN Eluent) (SB-806M HQ)
- Polyvinylpyrrolidone (6) (NaCl aq./CH3CN Eluent) (SB-806M HQ)
- Sodium Poly(Styrene Sulfonate) (1) (GF-510 HQ)
- Sodium Poly(Styrene Sulfonate) (2) (GF-7M HQ)
- Selection of Column (1) (Sodium Poly(Styrene Sulfonate))
- Selection of Column (2) (Sodium Polyacrylate)
- Selection of Column (3) (Poly(Sodium Methacrylate))
- Poly(Ethylene Glycol) Standards (6) (DE-613)
- Poly(Ethylene Glycol) Standards (7) (SB-2002.5)
- Poly(Ethylene Glycol) Standards (8) (SB-802.5 HQ)
- Sodium Dextran Sulfate (SB-806M HQ)
- Poly(Ethylene Glycol) Standards (9) (GS-220 HQ)
- Influence of Column Temperature on Retention Time of PEG (GF-310 HQ and GS-320 HQ)
- Comparison of Chromatogram of PEG Mixtures
- Influence of Salt Concentration on Retention Time of PEG with GF-310 HQ Column
- Effects of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent
- Effects of Analysis of PEG Mixtures with GS-320 HQ by Composition of Eluent
- SEC Analysis of Latex Particles (KW405-4F)
- Poly(Sodium Methacrylate) (GF-7M HQ)
Applications (Related Information)
- SEC Analysis of Ionic Sample
- Calibration Curves for SB-800 HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for SB-800 HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves for SB-806M HQ (Aqueous Eluent: Pullulan, PEG and PEO)
- Calibration Curve for SB-807 HQ (Aqueous Eluent: Pullulan)
- Calibration Curves for LB-800 Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GF-HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GF-HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves for GS-HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GS-HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves of Hydrophilic Polymers (1) (Sodium Poly(Styrene Sulfonate))
- Calibration Curves of Hydrophilic Polymers (2) (Sodium Polyacrylate)
- Calibration Curves of Hydrophilic Polymers (3) (Poly(Sodium Methacrylate))