A column, which has the most appropriated calibration curve for the sample molecular
weight range should be selected. For example, regarding the two columns (1) and
(2), whose calibration curves are shown in the figure, column (2) is best suited
for the analysis of the mixture of A, B and C. In other words, among the column
calibration curves which covers all the sample molecular weight range, a column
whose calibration curve has the smallest inclination should be selected to obtain
a good separation.
Applications
- Hydrophobic Polymers: THF Eluent
- Hydrophobic Polymers: Chloroform Eluent
- Hydrophobic Polymers: DMF Eluent
- Hydrophobic Polymers: HFIP Eluent
- Hydrophobic Polymers: Other Eluents
- Additives in Hydrophobic Polymers
- Hydrophobic Oligomers
- Hydrophilic Polymers
- Cationic Polymers
- Solvent-peak Separation Columns
Applications (Related Information)
- To Realize Pure SEC Mode
- Guidelines for Shodex Column Selection
- Sample Concentration for SEC Analysis
- Effects of Sample Load (Pullulan)
- Selection of Calibration Standards
- Connection of Different Pore-size Columns
- Comparison of Calibration Curves: Linear Type and Conventional Type
- Comparison of Separation: Linear Type and Conventional Type (1)
- Comparison of Separation: Linear Type and Conventional Type (2)
- Solubility of Solvents
- Physical Chemistry Properties of Solvents
- Polarities of Solvents