The α-linolenic acid is a functional ingredient in foods and is mainly found in edible blended oils. The α-linolenic acid is focused for its effects in reducing visceral fat, body weight, and BMI. Foods such as edible blended oil that contains high concentration of α-linolenic acid can be analyzed using a HPLC method. In this application, we analyzed α-linolenic acid in edible blended oil using Asahipak ODP-50 4D. The edible blended oil can be analyzed after a simple dilution. Using the method, α-linolenic acid was successfully detected and a linear calibration curve in the concentration range 10 to 1,000 mg/L was obtained.
(Sample Preparation)
1) Dilute a commercial edible blended oil 1+1 with THF without BHT.
2) Filter the mixture using a 0.45-µm membrane filter and use it as the injection sample.
Sample : 5 µL
1. α-Linolenic acid 1000 mg/L
(in THF without containing BHT)
Column : Shodex Asahipak ODP-50 4D (4.6 mm I.D. x 150 mm) Eluent : 0.1 % H3PO4 in (H2O/CH3CN=35/65) Flow rate : 1.0 mL/min Detector : UV (215 nm) Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Leukotrienes (ODP-50 6D)
- Leukotrienes Produced by Polymorphonuclear Leukocyte of Rat (ODP-50 6D)
- Hydroxyeicosatetraenoic Acid Produced by Polymorphonuclear Leukocyte of Rat (ODP-50 6D)
- Hydroxyeicosatetraenoic Acids (ODP-50 6D)
- Plasma Lipoproteins (1) (SB-805 HQ)
- Glycerides (1) (Chloroform Eluent) (GF-310 HQ)
- Glycerides (2) (THF Eluent) (GF-310 HQ)
- Glycerides (3) (DMF Eluent) (GF-310 HQ)
- Triglycerides (GF-310 HQ)
- Comparison of Triglycerides Separation
- Fatty Acids (6) (KF-402HQ)
- Unsaturated Fatty Acids (ODP-50 4D)
- Analysis of Fatty Acids with Corona CAD (DS-613)
- Fatty Acid Methyl Esters (DS-413)
- Analysis of Unsaturated Fatty Acids in Supplements (ODP-50 4D)
- Analysis of Coenzyme Q10 in a Supplement (C18M 4D)