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Analysis of Zanamivir According to USP Method (NH2P-50 4E)

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Zanamivir is a neuraminidase inhibitor and it is effective in the treatment of influenza caused by influenza A and B viruses. According to USP (United States Pharmacopeia) method, zanamivir should be analyzed using a column which can separate zanamivir and talo-zanamivir (internal standard) with resolution (Rs) of ≥ 1.5 and relative standard deviation (RSD) of ≤ 1.5%. It was confirmed that the all conditions were satisfied when zanamivir and talo-zanamivir were analyzed using Asahipak NH2P-50 4E.


Sample : 20μL
Zanamivir resolution solution
1. Zanamivir 0.05mg/mL
2. talo-Zanamivir 2.5μg/mL


Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : Acetonitrile and 7.5mM sulfuric acid (60:40).
               Adjust with ammonia TS to a pH of 6.2
Flow rate    : 1.5mL/min
Detector     : UV(234nm)
Column temp. : 30°C

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