Glucose can be anearobically decomposed into lactic acid and ethanol. This decomposing process is called as glucolytic pathway. In this pathway, some products such as glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, etc. are produced as intermediary products. Here, glucose-1-phosphate, fructose-6-phosphate and glucose-6-phosphate are analyzed using Asahipak NH2P-50 4E by ion-exchange mode. It was confirmed that the retention is stronger and separation is better by using lower concentration phosphate buffer.
Sample : (Left); 20 mg/mL each, (Right); 10 mg/mL each, 20 μL
1. Glucose-1-phosphate
2. Fructose-6-phosphate
3. Glucose-6-phosphate
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : (Left); 10 mM Sodium phosphate buffer (pH4.4) : (Right); 150 mM Sodium phosphate buffer (pH4.4) Flow rate : 1.0 mL/min Detector : RI Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Oligogalacturonic Acids (NH2P-50 4E)
- Analysis of Oligogalacturonic Acids (VN-50 4D)
- Phosphorylated Saccharides (2) (NH2P-50 4E)
- Phytic Acid (GS-220 HQ)
- Separation of N-acetylneuraminic Acid and N-glycolyneuraminic Acid (KC-811)
- Analysis of Glucuronic Acid and Glucuronolactone (KC-811)
- Separation of Glucuronolactone and Organic Acids (KC-811)
- LC/MS Analysis of Inositol Phosphates (VG-50 2D)