LC/MS Analysis of Organic Acids (DE-213)

A polymer-based reversed phase column, RSpak DE-213, was used with a LC/MS to separate organic acids.


Sample : 50 ng/mL each, 10 μL
Glyoxylic acid
Pyruvic acid
Glycolic acid
Lactic acid
Maleic acid
Succinic acid
Fumaric acid
Adipic acid

Column       : Shodex RSpak DE-213 (2.0 mm I.D. x 150 mm)
Eluent       : (A); 0.1 %(v/v) Formic acid aq.
               (B); CH3CN
               Linear gradient: 
               (B %) 5 % (0 min) -> 5 % (2 min) -> 15 % (2.5 min) -> 15 % (10 min)
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM Negative)
Column temp. : 30 °C

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