Elution Volume of Organic Acids (NN-814)

Elution volumes of organic acids using RSpak NN-814 are shown in the table.

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Substances Elution Volume (mL) Substances Elution Volume (mL)
Oxalic acid   7.6 Lactic acid 10.2
Maleic acid   8.8 Formic acid 10.2
D-Glucuronic acid   8.9 Citraconic acid 10.7
Gluconic acid   9.0 Succinic acid 10.8
α-Ketoglutaric acid   9.2 Acetic acid 10.8
Tartaric acid   9.2 Levulinic acid 11.1
Pyruvic acid   9.4 Glutaric acid 11.7
Citric acid   9.5 Itaconic acid 12.3
Glyoxylic acid   9.7 Adipic acid 12.9
Malic acid   9.8 Fumaric acid 13.4
Glycolic acid   9.8 trans-Crotonic acid 15.2
Malonic acid 10.0 n-Caproic acid 30.8
Pyroglutamic acid 10.0 L-Glutamic acid 37.8
Column
Shodex RSpak NN-814 (8.0 mm I.D. x 250 mm)
Eluent
0.1 % H3PO4 aq.
Column temp.
60 ℃

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