Analysis of Organic Acids and Vitamin C in Fruit Juice (KC-811)

The main organic acids and Vitamin C contained in fruit are separated using RSpak KC-811 with 1 mM perchloric acid as the eluent.

Sample: Fruit juice

  1. 1.Cl-                 7.Succinic acid
  2. 2.Oxalic acid    8.Formic acid
  3. 3.Maleic acid    9.Acetic acid
  4. 4.Citric acid
  5. 5.Malic acid
  6. 6.Vitamin C
Column
Shodex RSpak KC-G 8B (8.0 mm I.D. x 50 mm) + KC-811 (8.0 mm I.D. x 300 mm) x 2
Eluent
1 mM HClO4 aq.
Flow rate
(Eluent); 1.0 mL/min, (Reagent); 0.4 mL/min
Detector
VIS (430 nm) *Post column method
Column temp.
40 ℃

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