RSpak NN-814, which has a higher polarity gel than that of RSpak KC-811 is suitable for the analysis of aromatic organic acids. The ion exclusion and reversed phase modes contribute to the separation of aromatic organic acids.
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| Substances | Elution Volume (mL) | Substances | Elution Volume (mL) |
|---|---|---|---|
| Shikimic acid | 10.1 | Benzoic acid | 51.0 |
| DL-Mandelic acid | 19.2 | Caffeic acid | 66.9 |
| Phthalic acid | 19.8 | Phenylpropionic acid | 68.4 |
| Trimellitic acid | 23.0 | Trimesic acid | 69.0 |
| m-Hydroxybenzonic acid | 46.5 | Salicylic acid | 71.4 |
| Vanillic acid | 47.1 | m-Toluic acid | 86.4 |
| p-Hydroxybenzoic acid | 47.6 | Ferulic acid | 87.0 |
| o-Hydroxybenzoic acid | 48.8 | p-Coumaric acid | 90.3 |
| 3-Phenylpropionic acid | 49.7 |
- Column
- ：Shodex RSpak NN-814 (8.0 mm I.D. x 250 mm)
- Eluent
- ：0.1 % H3PO4 aq.
- Column temp.
- ：60 ℃
RSpak NN-814, which has a higher polarity gel than that of RSpak KC-811 is suitable for the analysis of aromatic organic acids. The ion exclusion and reversed phase modes contribute to the separation of aromatic organic acids.
| Substance | Elution Volume (mL) | Substance | Elution Volume (mL) |
|---|---|---|---|
| Shikimic acid | 10.1 | Benzoic acid | 51.0 |
| DL-Mandelic acid | 19.2 | Caffeic acid | 66.9 |
| Phthalic acid | 19.8 | Phenylpropionic acid | 68.4 |
| Trimellitic acid | 23.0 | Trimesic acid | 69.0 |
| m-Hydroxybenzonic acid | 46.5 | Salicylic aci | 71.4 |
| Vanillic acid | 47.1 | m-Toluic acid | 86.4 |
| p-Hydroxybenzoic acid | 47.6 | Ferulic acid | 87.0 |
| o-Hydroxybenzoic acid | 48.8 | p-Coumaric acid | 90.3 |
| 3-Phenylpropionic acid | 49.7 |
Column : Shodex RSpak NN-814 (8.0 mm I.D. x 250 mm) Eluent : 0.1 % H3PO4 aq. Column temp. : 60 °C
Sample Name Index
- 3-Phenylpropionic acid
- Benzoic acid
- Caffeic acid
- Ferulic acid
- Mandelic acid
- Phthalic acid
- Salicylic acid
- Shikimic acid
- Trimellitic acid
- Trimesic acid
- Vanillic acid
- m-Toluic acid
- o-Hydroxybenzoic acid
- p-Coumaric acid
- p-Hydroxybenzoic acid
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Effects of Flow Rate (KC-811)
- Effects of Injection Volume (KC-811)
- Effects of Sample Load (KC-811)
- Effects of Column Temperature (KC-811)
- Effects of Concentration of Eluent on Elution Volume (KC-811)
- Effects of Column Temperature on Elution Volume (1) (KC-811)
- Effects of Column Temperature on Elution Volume (2) (KC-811)
- Effects of Acetonitrile in Eluent (1) (KC-811)
- Effects of Acetonitrile in Eluent (2) (KC-811)
- Effects of Reversed Phase Column on Elution Volume (KC-811 + DE-613)
- Elution Volume of Organic Acids (NN-814)
- Elution Volume of Organic Acids (DE-413)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Standards) (KC-811)
- Comparison of Analysis of Organic Acids with 4 Kinds of Detectors (Soy Sauce) (KC-811)
- Organic Acid Standards (1) (KC-811)
- Organic Acid Standards (2) (KC-811)
- Organic Acid Standards (3) (KC-811)
- Organic Acid Standards (4) (DE-413)
- Organic Acid Standards (5) (DE-213)
- Citric Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Beer (KC-811)
- Analysis of Organic Acids and Vitamin C in Fruit Juice (KC-811)
- Reverse Phase Analysis of Gluconic Acids (DE-613)
- Analysis of Organic Acids in Sake (Japanese Rice Wine) (KC-811)
- Analysis of Organic Acids in White Wine (KC-811)
- Analysis of Oxalic Acid Using Ion Exclusion Mode (KC-811)
- Organic Acid in Phosphoric Acid (DE-613 + KC-811)
- Analysis of Organic Acids in Soy Sauces (KC-811)
- Organic Acids in Vinegar (KC-811)
- Separation of Succinic Acid and Fumaric Acid (KC-811)
- Analysis of Organic Acids (Eluent Conteinig Cyclodextrin) (KC-811)
- Camphor and Benzoic Acid (NN-814)
- Di-carboxylic Acids (DE-413)
- Analysis of Organic Acids in Soybean Paste (KC-811)
- Analysis of Organic Acids in Soy Sauce For Noodles (KC-811)
- Organic Acids Analysis in Black-vinegar (KC-811)
- LC/MS Analysis of Organic Acids (DE-213)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- Organic Acids (KC-811)
- LC/MS Analysis of Pipecolic Acid (NH2P-40 2D)
- Elution Volume of Organic Acids (NI-424)
- Analysis of Formic Acid in Povidone According to Pharmacopeia Method (KC-811)
- Analysis of Related Compounds of Alanine and Aspartic Acid According to USP-NF Method (SH1011)
- Analysis of Acetic Acid in Vinegar Drinks (KC-811)
- Citric Acid Analysis in Functional Beverage (KC-811)
- Organic Acid Determination Test for Glycerin Fatty Acid Esters According to Japan’s Specifications and Standards for Food Additives (SH1011) (SH1011)