Absolute Molecular Weight Determination of Heparin (LB-806M)

Absolute molecular weight of heparin was determined by OHpak LB-806M, a column for aqueous SEC. LB-800 series are compatible with multi angle light scattering (MALS) detector. By minimizing the baseline noise level, LB-800 series enable MALS detector to detect low molecular weight compounds which are difficult to be detected by conventional columns.


Sample : 100 μL
0.1 % Sodium heparin


Columns      : Shodex OHpak LB-806M (8.0 mm I.D. x 300 mm) x 2
Eluent       : 0.1 M NaNO3 aq.
Flow rate    : 1.0 mL/min
Detector     : MALS (Multi angle light scattering), RI
Column temp. : 30 °C

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