Chitosan (SB-806M HQ)

Chitosan is a polysaccharide containing D-glucosamine and N-acetyl-D-glucosamine; it is obtained by partial deacetylation of chitin. Chitosan is used as a coating agent, a disintegrator, a binder etc. Acetic acid aqueous solution was used as the eluent, because chitosan hardly dissolves in water but easily dissolves in an organic acid aqueous solution. Chitosan has positive charge in acetic acid aqueous solution, and is adsorbed to the packing materials. Therefore, sodium nitrate was added to the eluent for reducing the ion adsorption.


Sample : 0.1 % each*, 100 µL
Chitosan (5 - 20 Pa・S)
Chitosan (50 - 100 Pa・S)
Chitosan (200 - 600 Pa・S)
*After dissolving sample with 50 mM acetic acid aq., add sodium nitrate to a final concentration of 0.3 M.


Columns      : Shodex OHpak SB-806M HQ (8.0 mm I.D. x 300 mm) x 2
Eluent       : 50 mM CH3COOH + 0.3 M NaNO3 aq.
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C

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