Taurine, sulfur containing amino acid, was analyzed using SUGAR KS-801, a column for saccharides analysis. Generally, columns used for ligand exchange chromatography are used with water as the eluent, but adding cations as the counter ion enables the columns to separate samples under ion exchange modes well. NaOH aqueous solution was used as an eluent for SUGAR KS-801 with the counter ion as Na+, allowing ligand exchange and ion exchange to occur. Ligand exchange occurred between the counter ions of the packing material and the hydroxy groups of saccharides. Ion exchange occurred between the sulfo groups and the amino groups of taurine. For sensitive measurement, attention should be paid to the positions of vacant peak due to the cations in the eluent.
Sample : 0.05 % each, 20 µL
1. Sucrose
2. myo-Inositol
3. Taurine
Column : Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm) Eluent : 10 mM NaCl aq. Flow rate : 1.0 mL/min Detector : RI Column temp. : 80 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Volume of Saccharides and Sugar Alcohols (NH2P-50 4E)
- Sugar Alcohols (1) (SC1211)
- Sugar Alcohols (2) (NH2P-50)
- Sugar Alcohols (3)
- Saccharides and Sugar Alcohols (1) (SZ5532)
- Saccharides and Sugar Alcohols (2) (SP0810)
- Saccharides and Sugar Alcohols (3) (SP0810)
- Saccharides and Sugar Alcohols (4) (SC1011)
- Saccharides and Sugar Alcohols (5) (SZ5532)
- Saccharides and Sugar Alcohols (6) (NH2P-50)
- Saccharides and Amino Acids (1) (SC1011)
- Saccharides and Amino Acids (2) (SC1211)
- Saccharides and Amino Sugars (2) (NH2P-50 4E)
- Saccharides and Amino Sugars (3) (SC1011)
- Xilitol in Tablet Candy
- Apple Juice (NH2P-50 4E)
- Apple Juice (SC1011)
- Sake (Japanese Rice Wine)
- Effects of Acetonitrile Concentration in Analysis of Sugar Alcohols
- Amino Sugars (SB-802.5 HQ)
- Soft Drink Containing Erythritol (NH2P-50 4E)
- Palatinit (SP0810, SC1011, SZ5532)
- Analysis of Pinitol (SP0810)
- Analysis of Meglumine (NN-814)
- LC/MS/MS Analysis of Meglumine (VC-50 2D)
- Analysis of Meglumine (YS-50)
- Separation of myo-Inositol and D-chiro-Inositol (SZ5532)
- Separation of Taurine and Inositol (NH2P-50 4E)
- Analysis of Hydrogenated Maltose Starch Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Mannitol According to Pharmacopeia Method (SC1011-7F)
- Analysis of myo-Inositol According to EP Method (SC1011-7F)
- Analysis of Xylitol According to USP-NF Method (SP0810)
- Analysis of Sorbitol According to USP-NF Method (SP0810 8C)
- Analysis of Maltitol According to USP-NF Method (SP0810 8C)
- Comparison of Sensitivity of NH2P Polymer-based Column and Silica-based Amino Column with ELSD
- Analysis of Erythrose and Erythritol (VG-50 4E)