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LC/MS/MS Analysis of Meglumine (VC-50 2D)

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Meglumine is an amino-sugar derived from sorbitol. It is often used in pharmaceuticals or radio contrast media. In this application, LC/MS/MS analysis of meglumine was completed using a column for HILIC mode: HILICpak VC-50 2D. Acidic conditions are suitable for high sensitive analysis under positive mode for amine compounds.


Sample : 5μL
1. Meglumine 1ng/mL (in H2O)


Column       : Shodex HILICpak VC-50 2D (2.0mmI.D. x 150mm)
Eluent       : 50mM HCOOH aq./CH3CN=60/40
Flow rate    : 0.2mL/min
Detector     : ESI-MS/MS (MRM Positive)
Column temp. : 40°C

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