When analyzing saccharides in food, it is necessary to remove the substances besides saccharides before the analysis. This procedure is called as sample pre-treatment. Here, the analysis result of saccharides in food using Asahipak NH2P-50 4E is shown together with the sample pre-treatment procedure.
(Sample pre-treatment for apple juice)
1) Put 5 g of apple juice in a 50 mL a volumetric flask.
2) Dilute with ultra pure water to make up a 30 mL solution.
3) Neutralize with 10 %(W/V) NaOH solution.
4) Apply ultrasonic vibration for 30 minutes using an ultrasonic vibrator.
5) Add ultra pure water to make up a 50 mL solution.
6) Filtrate the solution with a paper filter (No.5B).
7) Take a part of filtrated solution and add the same volume of acetonitrile.
8) Pass through a 0.45 μm membrane filter before injecting into HPLC.
Sample : Apple juice, 20 μL
1. Fructose
2. Sorbitol
3. Glucose
4. Sucrose
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : CH3CN/H2O=75/25 Flow rate : 1.0 mL/min Detector : RI Column temp. : Room temp.(25 °C)
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Volume of Saccharides and Sugar Alcohols (NH2P-50 4E)
- Sugar Alcohols (1) (SC1211)
- Sugar Alcohols (2) (NH2P-50)
- Sugar Alcohols (3)
- Saccharides and Sugar Alcohols (1) (SZ5532)
- Saccharides and Sugar Alcohols (2) (SP0810)
- Saccharides and Sugar Alcohols (3) (SP0810)
- Saccharides and Sugar Alcohols (4) (SC1011)
- Saccharides and Sugar Alcohols (5) (SZ5532)
- Saccharides and Sugar Alcohols (6) (NH2P-50)
- Saccharides and Amino Acids (1) (SC1011)
- Saccharides and Amino Acids (2) (SC1211)
- Saccharides and Amino Acids (3) (KS-801)
- Saccharides and Amino Sugars (2) (NH2P-50 4E)
- Saccharides and Amino Sugars (3) (SC1011)
- Xilitol in Tablet Candy
- Apple Juice (SC1011)
- Sake (Japanese Rice Wine)
- Effects of Acetonitrile Concentration in Analysis of Sugar Alcohols
- Amino Sugars (SB-802.5 HQ)
- Soft Drink Containing Erythritol (NH2P-50 4E)
- Palatinit (SP0810, SC1011, SZ5532)
- Analysis of Pinitol (SP0810)
- Analysis of Meglumine (NN-814)
- LC/MS/MS Analysis of Meglumine (VC-50 2D)
- Analysis of Meglumine (YS-50)
- Separation of myo-Inositol and D-chiro-Inositol (SZ5532)
- Separation of Taurine and Inositol (NH2P-50 4E)
- Analysis of Hydrogenated Maltose Starch Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Mannitol According to Pharmacopeia Method (SC1011-7F)
- Analysis of myo-Inositol According to EP Method (SC1011-7F)
- Analysis of Xylitol According to USP-NF Method (SP0810)
- Analysis of Sorbitol According to USP-NF Method (SP0810 8C)
- Analysis of Maltitol According to USP-NF Method (SP0810 8C)
- Comparison of Sensitivity of NH2P Polymer-based Column and Silica-based Amino Column with ELSD
- Analysis of Erythrose and Erythritol (VG-50 4E)