Elution volumes of saccharides using HILICpak VG-50 4E, a polymer-based amino column are shown in the table. The (4) eluent condition is recommended for analysis of aldoses. (Disaccharides is retained too long under the (4) eluent condition.)
As only the peak top position can be obtained from the table, if you want to to see the chromatogram with actual peak width, please contact us.
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| Substances | Elution Volume (mL) | |||
|---|---|---|---|---|
| Eluent (1) | Eluent (2) | Eluent (3) | Eluent (4) | |
| Arabinose | 7.36 | 6.54 | 5.65 | 12.80 |
| Arabitol | 8.17 | 7.14 | 6.05 | 9.08 |
| meso-Erythritol | 6.22 | 5.60 | 5.10 | 6.65 |
| Fructose | 8.66 | 7.53 | 6.19 | 12.57 |
| Fucose | 6.33 | 5.71 | 5.06 | 9.36 |
| Galactose | 11.16 | 9.42 | 7.37 | 19.20 |
| Gentiobiose | 36.57 | 27.17 | 14.52 | - |
| Glucose | 11.97 | 10.15 | 7.47 | 21.95 |
| myo-Inositol | 21.43 | 16.87 | 11.68 | - |
| Isomaltose | 37.29 | 28.23 | 14.41 | - |
| Lactitol | 28.87 | 21.48 | 13.28 | - |
| Lactose | 26.94 | 20.31 | 12.46 | - |
| Lactulose | 20.90 | 15.93 | 10.52 | - |
| Maltitol | 27.79 | 21.44 | 12.37 | - |
| Maltose | 29.93 | 23.38 | 12.71 | - |
| Mannitol | 11.27 | 9.61 | 7.35 | 12.63 |
| Mannose | 10.73 | 9.26 | 6.95 | 20.49 |
| Melezitose | 35.84 | 46.47 | 18.67 | - |
| Melibiose | 39.07 | 26.92 | 15.07 | - |
| Palatinit | 2 peaks | 2 peaks | 14.23 | - |
| Palatinose | 24.40 | 19.00 | 11.26 | - |
| Rhamnose | 6.00 | 5.49 | 4.87 | 9.33 |
| Ribose | 6.04 | 5.54 | 4.93 | 10.83 |
| Sorbitol | 10.48 | 8.92 | 7.18 | 12.11 |
| Sorbose | 9.28 | 8.08 | 6.79 | 14.55 |
| Sucrose | 22.16 | 17.46 | 10.69 | - |
| Talose | 7.90 | 7.03 | 5.75 | 15.71 |
| Trehalose | 37.98 | 29.74 | 14.36 | - |
| Trehalulose | 26.39 | 20.49 | 11.92 | - |
| Xylitol | 7.88 | 6.89 | 6.04 | 8.90 |
| Xylobiose | 12.59 | 10.31 | 7.83 | - |
| Xylose | 7.72 | 6.89 | 5.78 | 14.16 |
(-): Unverified
- Column
- ：Shodex HILICpak VG-50 4E (4.6 mm I.D. x 250 mm)
- Eluent
- ：(1) ; H2O/CH3CN=12/88
(2) ; H2O/CH3OH/CH3CN=10/5/85
(3) ; H2O/CH3CN=20/80
(4) ; (8 mM NaOH + 5 mM NaCl aq.)/CH3CN=18/82 - Flow rate
- ：1.0 mL/min
- Detector
- ：RI
- Column temp.
- ：(1), (2); 60 ℃
(3), (4); 40 ℃
Sample Name Index
- meso-Erythritol
- myo-Inositol
- Arabinose
- Arabitol
- Fructose
- Fucose
- Galactose
- Gentiobiose
- Glucose
- Isomaltose
- Lactitol
- Lactose
- Lactulose
- Maltitol
- Maltose
- Mannitol
- Mannose
- Melezitose
- Melibiose
- Palatinit, Isomalt
- Palatinose, Isomaltulose
- Rhamnose
- Ribose
- Sorbitol, Glucitol
- Sorbose
- Sucrose, Saccharose
- Talose
- Trehalose
- Trehalulose
- Xylitol
- Xylobiose
- Xylose
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Elution Volume of Saccharides
- Separation of Anomer
- Comparison of Amino Column with Amide Column
- Comparison of Columns with Different Counter Ions
- Combination of Columns with Different Counter Ions
- Effects of Free Base Ratio of Amino Group on Plate Number (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (NH2P-50 4E)
- Effects of Acetonitrile Concentration on Elution Time (SZ5532)
- Effects of Acetonitrile Concentration on Plate Number (NH2P-50 4E)
- Durability Against Acidic Solvents (NH2P-50 4E)
- Durability Against Alkaline Solvents (NH2P-50 4E)
- Durability Against Eluent Composition Change (NH2P-50 4E)
- Effects of Sample Solvent Composition (NH2P-50 4E)
- Effects of Sample Injection Volume (NH2P-50 4E)
- Effects of Flow Rate (SC1011)
- Effects of Temperature (1) (SC1011)
- Effects of Temperature (2)
- Calibration Curves for Saccharides (NH2P-50 4E)
- Calibration Curves for Saccharides (SZ5532)
- Comparison of NH2P with Silica-based Amino Column (1)
- Comparison of NH2P with Silica-based Amino Column (2)
- Monosaccharides (1) (NH2P-50 4E)
- Monosaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (1) (SP0810)
- Monosaccharides and Disaccharides (2) (NH2P-50 4E)
- Monosaccharides and Disaccharides (3) (NH2P-50 4E)
- Monosaccharides and Disaccharides (4) (SZ5532)
- Monosaccharides and Disaccharides (5) (VG-50 4E)
- Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)
- Saccharides in Agricultural Products (SC1011)
- Saccharides in Wood (1) (SP0810)
- Saccharides in Wood (2) (SP0810)
- Saccharides in Wood (3) (NH2P-50 4E)
- Palatinose in Food (SC1011)
- Worcester Sauce (KS-801)
- Yogurt: Sugar added (SP0810)
- Yogurt: Sugar added (NH2P-50 4E)
- Roast Sweet Potato (NH2P-50 4E)
- Roast Sweet Potato (SP0810)
- Soybean Flour (SP0810)
- Milk Coffee (NH2P-50 4E)
- Jelly (NH2P-50 4E)
- Saccharides in Bread (KS-801)
- Lactic acid Beverage and Fermented Milk (SH1011)
- Orange and Melon (SH1011)
- Orange Juice (SC1011)
- Chocolate Cake (NH2P-50 4E)
- Chocolate Cake (SZ5532)
- Maltose and Isomaltose (SZ5532)
- Saccharide Analysis Using Semi-micro Column (NH2P-50 2D)
- High Sensitive Analysis of Saccharides in powdered Milk (NH2P-50 4E)
- Glucose Derivatives (NH2P-50 4E)
- Extract of Wheat Rod (NH2P-50 4E)
- Effects of Organic Solvent in Eluent (NH2P-50 4E)
- Saccharides in Food (SC1011)
- Monosaccharides Composing Sugar Chain (SH1011)
- Maltose and Nigerose (NH2P-50 4E)
- Analysis of Ketohexose (SP0810)
- Cello-oligosaccharides and Ethanol (KS-802)
- Analysis of Rare Sugar (1) (SP0810)
- Analysis of Rare Sugar (2) (VG-50 4E)
- Galactinol (SC1011)
- Saccharides Separation in Presence of Na Salt (DC-613)
- Heptulose Separation (KS-801)
- Saccharides Analysis using Corona Charged Aerosol Detector (NH2P-50 4E)
- Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)
- Analysis of Saccharides and Furfurals (KS-801)
- Saccharides Analysis in Ionic Liquid (DC-613)
- Gulose (SP0810)
- Simultaneous Analysis of Lactose, Epilactose, and Lactulose (VG-50 4E)
- Analysis of Aldoses (VG-50 4E)
- Abnormal Behavior of Chromatogram of Saccharides Using a Pb Type Column for Ligand Exchange Chromatography
- Analysis of Fructose Glucose Syrup According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Maltose Starch Powder According to Japanese Pharmaceutical Excipients Method (KS-801)
- Analysis of Ribose According to USP-NF Method (KS-801)
- Analysis of Dextrose According to USP-NF Method (SC1011)
- Analysis of Tagatose According to USP-NF Method (SC1011)
- Analysis of Maltose According to USP-NF Method (KS-801)
- Analysis of Trehalose According to USP-NF Method (KS-801)
- Analysis of Maltose Hydrate According to JP Method (KS-801)
- Analysis of Trehalose According to JP Method (KS-801)
- Analysis of Lactulose According to JP Method (SP0810)
- Analysis of Ethyl α-D-Glucoside in Sake (VG-50 4E)
- Analysis of Erythrose and Threose (SC1011)
- Analysis of 2-Deoxy-D-glucose (SC1011)
- Analysis of 2-Deoxy-D-glucose (VG-50 4E)
- Analysis of Dextrose and Fructose in Cranberry Juice (NH2P-50 4E)