LC/MS Analysis of Low Molecular Weight Peptides (VC-50 2D)

Physiological functions of low molecular weight peptides (dipeptides, tripeptides) have been drawing attention in recent years. In this application, dipeptides and tripeptides were analyzed using HILICpak VC-50 2D, a polymer-based HILIC column, with LC/MS detection. The peptides containing basic amino acids tend to be  retained longer.


Sample : 5 μL
50 ng/mL each (in H2O)
Ser-Glu
Gly-Tyr
Gly-Gly-Leu
Thr-Gln
Gly-Gly-Ala
Gly-Gln
Arg-Asp
Gly-Gly-His


Column       : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A); 100 mM HCOOH aq. (B); CH3CN 
               Linear gradient (High pressure);
               (B %) 60 % (0 to 5 min)
                     60 % to 10% (5 to 6 min)
                     10 % (6 to 15 min)
                     60 % (15.1 to 20 min) 
Flow rate    : 0.3 mL/min
Detector     : ESI-MS (SIM Positive)
Column temp. : 40 °C

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