The imidazole dipeptides, anserine (dipeptide of histidine and β-alanine) and carnosine (dipeptide of 1-methylhistidine and β-alanine), are found in large concentrations in the meat of bonito, tuna, whale, and the breast meat of birds. The imidazole dipeptides show a strong antioxidant effect which is effective for reducing fatigue and anti-aging. In this application, carnosine, anserine, and their constituent amino acids were analyzed simultaneously using polymer-based amino column, Asahipak NH2P-50 4E. These five compounds can be separated completely within 20 minutes.

[Note] This analysis requires column equilibration.
For column equilibration: First introduce 50 mM Sodium dihydrogen phosphate aq. at 0.5 mL/min for 2 hours, and then switch to the eluent.


Sample : 20 μL
1. β-Alanine 200 μg/mL
2. 1-Methyl-L-histidine 2 μg/mL
3．L-Anserine 5 μg/mL
4. Histidine 5 μg/mL
5. L-Carnosine 5 μg/mL
6. Nitrate
(derived from L-Anserine Nitrate)

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : 50 mM NaH2PO4 aq./CH3CN=40/60
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 40 °C