Although Asahipak GS-HQ series is basically SEC columns, they permit different types of separation and analysis from conventional ones by use of a multimode which combines the hydrophobic interaction (RPC) and ionic interaction (IEX: ion exclusion). The GS-320 HQ column performs well in separating hydrophillic peptides, particularly acidic and basic peptides. As the columns can be used over a wide range of eluent pH because of being polymer-based column, they can be washed with alkaline solution leading to a longer column life, which is another feature of the column.
Sample: 20 µL
- 1.Glu-Ala-Glu 0.025 %
- 2.Arg-Asp 0.05 %
- 3.Gly-His-Lys 0.025 %
- 4.Arg-Pro-Lys-Pro 0.025 %
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| Peptides | MW | pI | Σf |
|---|---|---|---|
| Glu-Ala-Glu | 347 | 3.12 | 0.39 |
| Arg-Asp | 289 | 6.75 | 0.68 |
| Gly-His-Lys | 340 | 9.95 | 0.29 |
| Arg-Pro-Lys-Pro | 497 | 11.44 | 3.24 |
- PI: Isocratic point
Σf: Hydrophobic parameter
- Column
- ：Shodex Asahipak GS-320 HQ (7.5 mm I.D. x 300 mm)
- Eluent
- ：50 mM Ammonium acetate buffer (pH6.7)
- Flow rate
- ：0.5 mL/min
- Detector
- ：UV (220 nm)
- Column temp.
- ：30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.