Opioid peptides were analyzed using Asahipak ODP-50 6D by pre-column reaction method.
Sample :
1. Tyr-Gly-Gly-Phe
2. ME, Met-Enkephalin
3. LE, Leu-Enkephalin
4. LE-Arg
5. α-EDP, α-Endorphin
6. LE-NH2
7. DNP 1-7
8. [D-Ala2] LE-NH2
9. ME-Arg-Gly-Leu
10. γ-EDP
11. ME-Arg-Phe
12. DNP 1-8
13. β-NED, β-Neoendorphin
14. α-NED, β-NED-Lys
15. DNP 1-9
16. DNP 1-10
17. DNP-B
Column : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm) Eluent : (A); 0.1 M Borate buffer (pH9.5)/H2O/CH3CN=10/39/1 (B); 0.1 M Borate buffer (pH9.5)/H2O/CH3CN=2/3/5 Gradient:(A) to (B) Flow rate : (0 to 25 min); 0.8 mL/min, (25 to 55 min); 1.0 mL/min, (after 55 min); 0.8 mL/min Detector : Fluorescence (Ex. 330 nm, Em. 430 nm) Column temp. : Room temp.
Guo-Qing Zhang et al., Chem. Pharm. Bull., 39 (1991) 126.
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Standard Proteins (4) (C4P-50 4D)
- Comparison of Silica-based Column with Polymer-based Column
- Proteins and Peptides (ODP-50 6D)
- Recovery of Peptides (ODP-50 6D)
- Basic Peptides
- Effects of Eluent pH (ODP-50 6D)
- Analysis of Oligopeptides with Acidic and Alkaline Eluent (ODP-50 6D)
- Oligopeptides (1)
- Oligopeptides (2)
- Dipeptides (DE-613)
- Angiotensins
- Opioid Peptides (1) (By Post-column Reaction Method)
- Tryptic Digest of Myoglobin (2) (ODP-50)
- Analysis of Glutathiones (By Post-column Reaction Method)
- Analysis of Lactoferrin Supplement (C4P-50 4D)
- Analysis of Lactoferrin Concentrate According to Analytical Methods of Food Additives in Foods (C4P-50 4D)