PROTEIN LW-403 4D is an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins. PROTEIN LW-403 4D and PROTEIN LW-803 (a standard column) were compared for the chromatogram of IgG. The analysis time using LW-403 4D can be completed in half the time compared to LW-803.
Sample : IgG from human serum 10 mg/mL
(LW-403 4D) 0.5 μL
(LW-803) 5 μL
(D) Dimer
(M) Monomer
Columns : Shodex PROTEIN LW-403 4D (4.6 mm I.D. x 150 mm) Shodex PROTEIN LW-803 (8.0 mm I.D. x 300 mm) Eluent : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl Flow rate : (LW-403 4D) 0.35 mL/min (LW-803) 1.0 mL/min Detector : (LW-403 4D) UV (280 nm) (small cell volume) (LW-803) UV (280 nm) (conventional type) Column temp. : Room temp.
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.