A calibration curve for PROTEIN LW-403 4D, an aqueous SEC (GFC) semi-micro column for rapid analysis of antibody proteins, using proteins is shown.


Sample :
Thyroglobulin (MW : 670,000)
γ-Globulin (MW : 160,000)
BSA (MW : 66,500)
Ovalbumin (MW : 45,000)
Myoglobin (MW : 17,000)
Ribonuclease A (MW : 13,000)
Aprotinin (MW : 6,500)

Column       : Shodex PROTEIN LW-403 4D (4.6 mm I.D. x 150 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 0.35 mL/min
Detector     : UV (280 nm) (small cell volume)
Column temp. : Room temp.