Various peptides with MW ranged from 269 to 1734 was analyzed using aqueous solvent SEC (GFC) high performance semi-micro column, KW402.5-4F. Since each peptide may contain different amino-functional groups, the separation mode occur may not be as a “pure Esize exclusion mode like for the analysis of proteins. This is more significant when peptides contain more hydrophobic-amino acids and/or basic-amino acids.


Sample : 5 μL
1. Gly-Gly-His
2. Thrombin receptor activator peptide (Ser-Phe-Leu-Leu-Arg-Asn-Pro-Asn-Asp-Lys-Tyr-Glu-Pro-Phe)
3. Angiotensin Ⅱ (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe)
4. Angiotensin Ⅰ (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu)
5. Leu-Enkephalin (Tyr-Gly-Gly-Phe-Leu)

Column       : Shodex KW402.5-4F (4.6 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 0.33 mL/min 
Detector     : UV (220 nm) 
Column temp. : 30 °C