Comparison of Chromatograms of Protein mixtures

This figure shows the chromatogram of protein mixtures analyzed with OHpak SB-803 HQ, Asahipak GF-510 HQ or PROTEIN KW-803. KW-803 is the best column for the separetion of these proteins.

Sample:

  1. A:Thyroglobulin (bovine)
  2. B:γ-Globulin (bovine)
  3. C:Ovalbumin (chiken)
  4. D:Myoglobin (horse)
  5. E:Cyanocobalamin
Columns
Shodex OHpak SB-803 HQ, Shodex PROTEIN KW-803 (8.0 mm I.D. x 300 mm each), Shodex Asahipak GF-510 HQ (7.5 mm I.D. x 300 mm)
Eluent
0.2 M Phosphate buffer (pH6.9)
Flow rate
0.5 mL/min
Detector
UV (280 nm)
Column temp.
30 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index

Applications

Applications (Related Information)