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Cation Exchange Chromatography Columns

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SP-825
CM-825
· Suitable for analyzing of relatively high molecular weight compounds: proteins, peptides, DNA, and RNA
· Usable in a wide pH range from pH 2 to 12
SP-420N · Non-porous base material
· For rapid analysis
New SP-FT 4A · Non-porous base material
· Provides ultra-rapid analysis using conventional devices
ES-502C 7C · Compared to IEC series columns, polyvinyl alcohol is used as base material and this offers different separation pattern
· Low hydrophobic interaction with proteins allows analysis under mild conditions
P-421S · Column for amino acids analysis by cation exchange mode
· Supports simultaneous analysis of different amino acids
· Fulfills USP L22 and L58 requirements


· Strong cation exchange resin (Functional group: Sulfopropyl)
(Standard columns) 

Product
code
Product nameIon exchange
capacity (meq/g)
Particle size
(μm)
Size (mm)
I.D. x Length
Shipping solvent
F6118250
IEC SP-825 0.4 8
8.0 x 75
50mM Na2SO4 aq.
F6113000
IEC SP-420N 0.3 2.5
4.6 x 35
20mM Sodium acetate buffer
+ 0.5M Na2SO4(pH5.0)
F6113100
New
IEC SP-FT 4A
0.2 2.7
4.6 x 10
20mM *MES buffer(pH5.6)
Product
name
Max. usable
pressure (MPa)
Max. usable
flow rate
(mL/min)
Usable temp.
range (°C)
Usable
pH range
Usable organic
solvent conc.
Usable
salt conc.
SP-825 2.0 1.5 10 to 50 2 to 12 ≤ 20% 20mM to 1.0M
SP-420N 20 2.0 0 to 60 2 to 12 ≤ 20% ≤ 1.5M
SP-FT 4A **20 3.0 5 to 45 2 to 12 ≤ 20% ≤ 1.5M

Housing Material of SP-FT 4A: PEEK
*MES: 2-(N-Morpholino)ethanesulfonic acid
**Total system pressure (system pressure and column pressure) varies depending on the system, solvent composition, temperature, and gradient conditions used. Use the column within the stated total system max pressure.

(Preparative columns) *Preparative columns are made to order.

Product codeProduct nameParticle size
(μm)
Size (mm)
I.D. x Length
Sandard column
F6548002
F6709604

IEC SP-2025
IEC SP-G 8B
(IEC SP-LG)
20
20

20.0 x 150
8.0 x 50

SP-825
(guard column)

· Weak cation exchange resin (Functional group: Carboxymethyl)
(Standard columns)

Product
code
Product nameIon exchange
capacity (meq/g)
Particle size
(μm)
Size (mm)
I.D. x Length
F6110002
IEC CM-825 0.4 8
8.0 x 75
F7640001
Asahipak ES-502C 7C 0.55 9
7.5 x 100
Product
name
Max. usable
pressure (MPa)
Max. usable
flow rate
(mL/min)
Usable temp.
range (°C)
Usable
pH range
Usable organic
solvent conc.
Usable
salt conc.
CM-825 2.0 1.5 10 to 50 2 to 12 ≤ 20% 20mM to 1.0M
ES-502C 7C 1.2 1.5 10 to 50 2 to 12 ≤ 100% 20mM to 0.6M
Product nameShipping solvent
CM-825
50mM Na2SO4 aq.
ES-502C 7C
0.1M Sodium phosphate buffer(pH4.4)

(Preparative columns) *Preparative columns are made to order.

Product codeProduct nameParticle size
(μm)
Size (mm)
I.D. x Length
Sandard column
F6548003
F6709605

IEC CM-2025
  IEC CM-G 8B
(IEC CM-LG)
20
20

20.0 x 150
8.0 x 50

CM-825
(guard column)

F6840003
F6710021
Asahipak ES-502C 20C
Asahipak GS-20G 7B
13
20
20.0 x 100
7.5 x 50
ES-502C
(guard column)

·For amino acids analysis
(Standard column)

Product codeProduct namePlate number
(TPN/column)
Functional
group
Particle size
(μm)
Size (mm)
I.D. x Length
F6354211
F6700210
CXpak P-421S
CXpak P-G
≥ 3,500
Guard column
Sulfo (Na+)
Sulfo (Na+)
6
6
4.6 x 150
4.6 x 10
Product
name
Max. usable
pressure (MPa)
Max. usable
flow rate
(mL/min)
Usable temp.
range (°C)
Usable
pH range
Usable organic
solvent conc.
Shipping
solvent
P-421S
P-G
5.0
-
0.7
-
15 to 63
15 to 63
≥ 3
≥ 3
≤ 15%
≤ 15%
H2O
H2O

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