糖和有机酸的洗脱体积 (SH1011)

SUGAR SH1011分析糖和有机酸的洗脱体积如下。当流动相硫酸浓度改变时，有机酸的洗脱体积也在一定程度上发生改变， 糖的洗脱体积改变相对较小。根据所要分析样品来选择硫酸的最佳浓度。

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Saccharides
Sugar alcohols		 Retention Time (min) Ratio of retention time
(b/a %)
Eluent (a) Eluent (b)
Stachyose   8.59   8.66 100.85
Isomaltotriose   8.85   8.90 100.59
Panose   8.94   8.98 100.48
Maltotriose   9.03   9.09 100.65
Gentiobiose   9.47   9.51 100.42
Isomaltose     9.63   9.69 100.64
Melibiose     9.77   9.82 100.51
Kojibiose   9.79   9.83 100.44
Maltose   9.80   9.86 100.60
Trehalose   9.82   9.87 100.53
Nigerose   9.87   9.87   99.99
Palatinose   9.96   9.75   97.91
Palatinit   9.96 10.04 100.77
Trehalulose   9.97 10.02 100.51
Lactose   9.99 10.05 100.55
Xylobiose 10.09 10.15 100.52
Maltitol 10.19 10.27 100.78
Lactitol 10.45 10.54 100.84
Glucose 11.32 11.37 100.47
Sorbose 11.54 11.58 100.32
myo-Inositol 11.69 11.75 100.47
D-Mannose 11.85 11.90 100.44
D(+)-Galactose 11.89 11.94 100.44
D(+)-Xylose 11.92 11.97 100.43
D(-)-Fructose 12.01 12.05 100.37
Mannitol 12.26 12.34 100.63
D(-)-Sorbitol 12.42 12.47 100.39
D(+)-Rhamnose 12.46 12.51 100.41
D(+)-Arabinose 12.74 12.79 100.40
D(-)-Ribose 13.01 13.04 100.22
D-Arabitol 13.07 13.14 100.57
Xylitol 13.25 13.33 100.61
D(+)-Fucose 13.35 13.40 100.40
meso-Erythritol 13.81 13.89 100.60
N-Acetyl-α-D-glucosamine 14.02 14.05 100.21
N-Acetyl-α-D-galactosamine 15.47 15.49 100.12
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Organic Acids Retention Time (min) Ratio of retention time
(b/a %)
Eluent (a) Eluent (b)
Oxalic acid   8.46   9.38 110.90
D-Glucuronic acid 10.24 10.39 101.46
Citric acid 10.25 10.48 102.26
α-Ketoglutaric acid 10.27 11.47 111.66
Maleic acid 10.28 11.77 114.46
N-Acetylneuraminic acid 10.53 10.94 103.89
Tartaric acid 10.67 11.06 103.65
Pyruvic acid 11.48 12.79 111.36
Malic acid 11.74 11.93 101.63
Malonic acid 11.95 12.53 104.84
trans-Aconitic acid 12.01 12.51 104.10
Succinic acid 13.78 13.83 100.39
DL-Lactic acid 14.73 14.86 100.85
Formic acid 15.70 15.82 100.78
Acetic acid 16.90 16.93 100.18
Adipic acid 17.32 17.42 100.59
Mesaconic acid 18.28 19.02 104.07
L-Pyroglutamic acid 19.00 19.53 102.77
Propionic acid 19.45 19.54 100.40
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Alcohol Retention Time (min) Ratio of retention time
(b/a %)
Eluent (a) Eluent (b)
Ethanol 23.43 23.52 100.38
Column
：Shodex SUGAR SH1011 (8.0 mm I.D. x 300 mm)
Eluent
：(a); 5 mM H2SO4 aq.
(b); 20 mM H2SO4 aq.
Flow rate
：0.6 mL/min
Detector
：RI
Column temp.
：60 ℃

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