使用三根不同的色谱柱RSpak DM-614, NN-814和DE-613来分析鸟嘌呤和色氨酸。



Sample : 1. Guanine , 2. Tryptophan

Columns     : (left);   Shodex RSpak DM-G 4A (4.6 mm I.D. x 10 mm)
                        + DM-614 (6.0 mmI.D. x 150 mm)
              (Center); Shodex RSpak NN-G (6.0 mm I.D. x 50 mm)
                        + NN-814 (8.0 mm I.D. x 250 mm)
              (Right);  Shodex RSpak DE-G 4A (4.6 mmI.D. x 10 mm)
                        + DE-613 (6.0 mm I.D. x 150 mm)
Eluent      : (Left);   0.3 M K2HPO4 (pH8.6)  
              (Center); 0.1 M NaH2PO4 (pH3.0 adjusted by H3PO4)
              (Right);  0.23 M K2HPO4 aq./CH3OH=80/20
Flow rate   : (Left); 0.8 mL/min, (Center); 1.0 mL/min, (Right); 0.8 mL/min
Detector    : (Left); UV (278 nm), (Center); RI, (Right); UV (278 nm)
Column temp.: (Left); 30 ℃, (Center); 50 ℃, (Right); 30 ℃